Antitumor effect of membrane-type Tim-3 on hepatocellular carcinoma Hepa1-6 cells of ICR mice
- Ju Liu
- Haiying Sun
- Dan Shen
- Mingmin Wang
- Zirong Wen
Published online on: December 14, 2017
In the present study, the inhibitory effect of transmembrane Tim‑3 on hepatocellular carcinoma Hepa1‑6 cells and the potential application of Tim‑3 on immune system of Institute of Cancer Research (ICR) mice loaded with Hepa1‑6 hepatocellular carcinoma was investigated. The animal model was established via inoculation of Hepa1‑6 hepatocarcinoma cells at the hind thigh of ICR mice. Recombinant vector plasmids were transfected at the same site for gene therapy by injection to observe the inhibitory effect of Tim‑3 on tumor growth. A panel of genes from tumor tissues at various time intervals was analyzed by reverse transcription‑polymerase chain reaction. Flow cytometry was used to evaluate the proliferation and cytotoxicity of splenocytes after Tim‑3 transfection. Synergistic effects of Tim‑3 with tumor abnormal protein‑1 (TAP1) was also studied. It was revealed that the growth of tumor was significantly suppressed after the transfection of Tim‑3. In the presence of Tim‑3, the proliferation of splenocytes and cytolysis in the early phase of tumor development was significantly enhanced, and this antitumor effect was further improved by the synergistic effect of Tim‑3 with TAP1. Therefore, the membrane‑type Tim‑3 may behave as an effective immunoregulator to enhance antitumor immune responses. Furthermore the present findings suggest that antitumor immunity was improved by the combined effect of Tim‑3 and TAP1.