Open Access

High expression of PRPS1 induces an anti-apoptotic effect in B-ALL cell lines and predicts an adverse prognosis in Chinese children with B‑ALL

  • Authors:
    • Yimei Ma
    • Xizhou An
    • Xianmin Guan
    • Qinglin Kong
    • Yanzhen Wang
    • Pengfei Li
    • Yan Meng
    • Yinghui Cui
    • Xianhao Wen
    • Yuxia Guo
    • Yali Shen
    • Jie Yu
  • View Affiliations

  • Published online on: January 29, 2018     https://doi.org/10.3892/ol.2018.7903
  • Pages: 4314-4322
  • Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Phosphoribosyl pyrophosphate synthetase 1 (PRPS1) is closely associated with a number of diseases; however, its influence in B-cell acute lymphoblastic leukemia (B‑ALL) and the potential molecular mechanisms involved remain unclear. The present study aimed to evaluate the expression of PRPS1 in Chinese children with B‑ALL and to investigate the mechanism of action of PRPS1 in this disease. A Cell Counting Kit‑8 (CCK‑8) assay was performed to examine the proliferation of B‑ALL Sup‑B15 and Raji cells, and flow cytometric analysis was conducted to determine the cell cycle distribution and rate of apoptosis. The mRNA and protein expression levels of PRPS1, MYC proto‑oncogene, bHLH transcription factor, cyclin E1, B‑cell lymphoma‑2 (Bcl‑2), cyclin dependent kinase 2 and caspase‑3 were detected by reverse transcription‑quantitative polymerase chain reaction and western blot analysis, respectively. Elevated PRPS1 expression was associated with a high‑risk stratification and poor prognosis in patients with B‑ALL. Furthermore, overexpression of PRPS1 accelerated the growth of and inhibited apoptosis in Sup‑B15 and Raji cells as well as increasing the expression of Bcl‑2 to induce an anti‑apoptotic effect in B‑ALL cell lines. The results of the present study indicate that PRPS1 regulates multiple processes in B‑ALL and may be an attractive therapeutic target.

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April 2018
Volume 15 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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APA
Ma, Y., An, X., Guan, X., Kong, Q., Wang, Y., Li, P. ... Yu, J. (2018). High expression of PRPS1 induces an anti-apoptotic effect in B-ALL cell lines and predicts an adverse prognosis in Chinese children with B‑ALL. Oncology Letters, 15, 4314-4322. https://doi.org/10.3892/ol.2018.7903
MLA
Ma, Y., An, X., Guan, X., Kong, Q., Wang, Y., Li, P., Meng, Y., Cui, Y., Wen, X., Guo, Y., Shen, Y., Yu, J."High expression of PRPS1 induces an anti-apoptotic effect in B-ALL cell lines and predicts an adverse prognosis in Chinese children with B‑ALL". Oncology Letters 15.4 (2018): 4314-4322.
Chicago
Ma, Y., An, X., Guan, X., Kong, Q., Wang, Y., Li, P., Meng, Y., Cui, Y., Wen, X., Guo, Y., Shen, Y., Yu, J."High expression of PRPS1 induces an anti-apoptotic effect in B-ALL cell lines and predicts an adverse prognosis in Chinese children with B‑ALL". Oncology Letters 15, no. 4 (2018): 4314-4322. https://doi.org/10.3892/ol.2018.7903