Open Access

Polysaccharide peptide isolated from grass-cultured Ganoderma lucidum induces anti-proliferative and pro-apoptotic effects in the human U251 glioma cell line

  • Authors:
    • Chunhua Wang
    • Dongmei Lin
    • Quan Chen
    • Shuqian Lin
    • Songsheng Shi
    • Chunmei Chen
  • View Affiliations

  • Published online on: January 19, 2018     https://doi.org/10.3892/ol.2018.7823
  • Pages:4330-4336
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The Ganoderma lucidum (G. lucidum) mushroom is one of the most extensively studied functional foods, known for its numerous health benefits, including the inhibition of tumor cell growth. The present study assessed the anti-proliferative and pro‑apoptotic activity of a novel G. lucidum polysaccharide peptide (GL‑PP) in human glioma U251 cells, which was purified from grass‑cultured G. lucidum. GL‑PP is a glycopeptide with an average molecular weight of 42,635 Da and a polysaccharide‑to‑peptide ratio of 88.70:11.30. The polysaccharides were composed of l‑arabinose, d‑mannose and d‑glucose at a molar ratio of 1.329:0.372:2.953 and a total of 17 amino acids were detected. The results of the current study demonstrated that GL‑PP significantly inhibited U251 cellular proliferation. The proportion of G0/G1 phase cells and sub‑G1 phase cells significantly increased as the concentration of GL‑PP increased, as did the activity of caspase-3. These results indicate that GL‑PP directly inhibited human glioma U251 proliferation by inducing cell cycle arrest and promoting apoptosis.

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April 2018
Volume 15 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Wang, C., Lin, D., Chen, Q., Lin, S., Shi, S., & Chen, C. (2018). Polysaccharide peptide isolated from grass-cultured Ganoderma lucidum induces anti-proliferative and pro-apoptotic effects in the human U251 glioma cell line. Oncology Letters, 15, 4330-4336. https://doi.org/10.3892/ol.2018.7823
MLA
Wang, C., Lin, D., Chen, Q., Lin, S., Shi, S., Chen, C."Polysaccharide peptide isolated from grass-cultured Ganoderma lucidum induces anti-proliferative and pro-apoptotic effects in the human U251 glioma cell line". Oncology Letters 15.4 (2018): 4330-4336.
Chicago
Wang, C., Lin, D., Chen, Q., Lin, S., Shi, S., Chen, C."Polysaccharide peptide isolated from grass-cultured Ganoderma lucidum induces anti-proliferative and pro-apoptotic effects in the human U251 glioma cell line". Oncology Letters 15, no. 4 (2018): 4330-4336. https://doi.org/10.3892/ol.2018.7823