Knockdown of EIF5A2 inhibits the malignant potential of non-small cell lung cancer cells

  • Authors:
    • Cheng Chen
    • Bojia Zhang
    • Shanshan Wu
    • Yongxiang Song
    • Jian Li
  • View Affiliations

  • Published online on: January 22, 2018     https://doi.org/10.3892/ol.2018.7832
  • Pages:4541-4549
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Abstract

Eukaryotic translation initiation factor 5A2 (EIF5A2) has been demonstrated to be upregulated in numerous types of human cancer and is associated with cancer progression. However, the expression and role of EIF5A2 in non‑small cell lung cancer (NSCLC) remains unclear. In the present study, the role of EIF5A2 in NSCLC was investigated, in addition to the underlying molecular mechanisms by which EIF5A2 acts. Relative EIF5A2 expression levels were determined in NSCLC cells and compared with levels in non‑cancerous lung tissues. Short interfering (si)RNA targeted against EIF5A2 was used to knock down EIF5A2 levels in NSCLC cells. Cell proliferation, apoptosis rate, migration ability and invasion ability were determined in untreated and siRNA‑treated NSCLC cells, in addition to the relative protein expression levels of various tumorigenic proteins and E‑cadherin. EIF5A2 expression was significantly higher in NSCLC tissues compared with adjacent normal tissues. Knockdown of EIF5A2 in the NSCLC cells significantly inhibited cell proliferation and induced apoptosis. Furthermore, EIF5A2 silencing suppressed cell migratory and invasive capacities in vitro. Silencing of EIF5A2 in the NSCLC cells resulted in the downregulation of the tumorigenic proteins, apoptosis regulator Bcl‑2 and myc proto‑oncogene protein, and upregulation of E‑cadherin, suggesting that EIF5A2 promotes proliferation and metastasis through these proteins. EIF5A2 may therefore serve as a novel therapeutic target for the treatment of NSCLC.

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April 2018
Volume 15 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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APA
Chen, C., Zhang, B., Wu, S., Song, Y., & Li, J. (2018). Knockdown of EIF5A2 inhibits the malignant potential of non-small cell lung cancer cells. Oncology Letters, 15, 4541-4549. https://doi.org/10.3892/ol.2018.7832
MLA
Chen, C., Zhang, B., Wu, S., Song, Y., Li, J."Knockdown of EIF5A2 inhibits the malignant potential of non-small cell lung cancer cells". Oncology Letters 15.4 (2018): 4541-4549.
Chicago
Chen, C., Zhang, B., Wu, S., Song, Y., Li, J."Knockdown of EIF5A2 inhibits the malignant potential of non-small cell lung cancer cells". Oncology Letters 15, no. 4 (2018): 4541-4549. https://doi.org/10.3892/ol.2018.7832