Open Access

Expression profiling analysis of autophagy-related genes in perineural invasion of cutaneous squamous cell carcinoma

  • Authors:
    • Li‑Qiang Zheng
    • Shan‑Yi Li
    • Cheng‑Xin Li
  • View Affiliations

  • Published online on: February 6, 2018     https://doi.org/10.3892/ol.2018.7971
  • Pages:4837-4848
  • Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to identify the potential autophagy‑related genes and to explore the underlying molecular mechanisms involved in cutaneous squamous cell carcinoma of head and neck (cSCCHN) by bioinformatics analysis. The Gene Expression Omnibus (GEO) series GSE86544 was downloaded from the GEO database. The primary data was generated from cSCCHN with clinical perineural invasion (PNI) and cSCCHN without PNI, and was further analyzed in order to identify differentially expressed genes (DEGs). The results revealed 239 autophagy‑related DEGs. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed and intersected to investigate the predicted functions of the key DEGs, including hypoxia‑inducible factor 1α (HIF1A), mitogen‑activated protein kinase 8 (MAPK8), mammalian target of rapamycin (mTOR) and B‑cell lymphoma 2 like 1 (BCL2L1). Up and downregulated genes shared one pathway, namely ‘pathways in cancer’. Next, the protein‑protein interaction (PPI) network of the autophagy‑related DEGs was constructed using Cytoscape 3.30 software. HIF1A, MAPK8, mTOR and BCL2L1 were key nodes in the PPI network. Additionally, RAB23 gene expression was positively correlated with HIF1A, MAPK8 and ADP ribosylation factor GTPase activating protein 1 (ARFGAP1), but negatively correlated with mTOR and BCL2L1. The present results suggested that the genes HIF1A, MAPK8, mTOR, BCL2L1 and RAB23 may be associated with and serve as potential therapeutic targets in cSCCHN with clinical PNI.

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April 2018
Volume 15 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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APA
Zheng, L., Li, S., & Li, C. (2018). Expression profiling analysis of autophagy-related genes in perineural invasion of cutaneous squamous cell carcinoma. Oncology Letters, 15, 4837-4848. https://doi.org/10.3892/ol.2018.7971
MLA
Zheng, L., Li, S., Li, C."Expression profiling analysis of autophagy-related genes in perineural invasion of cutaneous squamous cell carcinoma". Oncology Letters 15.4 (2018): 4837-4848.
Chicago
Zheng, L., Li, S., Li, C."Expression profiling analysis of autophagy-related genes in perineural invasion of cutaneous squamous cell carcinoma". Oncology Letters 15, no. 4 (2018): 4837-4848. https://doi.org/10.3892/ol.2018.7971