Open Access

Characterization of human gastric adenocarcinoma cell lines established from peritoneal ascites

  • Authors:
    • Bożenna Mytar
    • Małgorzata Stec
    • Rafał Szatanek
    • Kazimierz Węglarczyk
    • Katarzyna Szewczyk
    • Antoni Szczepanik
    • Grażyna Drabik
    • Jarek Baran
    • Maciej Siedlar
    • Monika Baj‑Krzyworzeka
  • View Affiliations

  • Published online on: February 8, 2018     https://doi.org/10.3892/ol.2018.7995
  • Pages: 4849-4858
  • Copyright: © Mytar et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The three cell lines, designated as gastric cancer (GC)1401, GC1415 and GC1436 were derived from peritoneal effusions from patients with gastric adenocarcinoma. Cell lines were established in tissue culture and in immunodeficient, non‑obese diabetic/severe combined immunodeficiency (NOD/SCID) mice. All cell lines were cultured in Dulbecco's modified Eagle's medium supplemented with 5% fetal bovine serum. These cell lines were grown as an adherent monolayer with doubling time ranging between 25 h (GC1436 cell line) and 30‑34 h (GC1401 and GC1415, respectively). All cells showed morphological features of epithelial‑like cells, forming sheets of polygonal cells. Chromosomal analysis showed that the modal numbers ranged from 52 (GC1401), 51‑56 (GC1415) and 106 (GC1436). High heterogeneity, resulting from several structural and numerical chromosomal abnormalities were evident in all cell lines. The surface marker expression suggested a tumor origin of the cells, and indicated the intestinal phenotype of a GC (CD10+, MUC1). All three cell lines were tumorigenic but not metastatic, in vivo, in NOD/SCID mice. The lack of metastatic potential was suggested by the lack of aldehyde dehydrogenase 1A1 activity. In conclusion, these newly established GC cell lines widen the feasibility of the functional studies on biology of GC as well as drug testing for potential therapeutic purposes.

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April 2018
Volume 15 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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APA
Mytar, B., Stec, M., Szatanek, R., Węglarczyk, K., Szewczyk, K., Szczepanik, A. ... Baj‑Krzyworzeka, M. (2018). Characterization of human gastric adenocarcinoma cell lines established from peritoneal ascites. Oncology Letters, 15, 4849-4858. https://doi.org/10.3892/ol.2018.7995
MLA
Mytar, B., Stec, M., Szatanek, R., Węglarczyk, K., Szewczyk, K., Szczepanik, A., Drabik, G., Baran, J., Siedlar, M., Baj‑Krzyworzeka, M."Characterization of human gastric adenocarcinoma cell lines established from peritoneal ascites". Oncology Letters 15.4 (2018): 4849-4858.
Chicago
Mytar, B., Stec, M., Szatanek, R., Węglarczyk, K., Szewczyk, K., Szczepanik, A., Drabik, G., Baran, J., Siedlar, M., Baj‑Krzyworzeka, M."Characterization of human gastric adenocarcinoma cell lines established from peritoneal ascites". Oncology Letters 15, no. 4 (2018): 4849-4858. https://doi.org/10.3892/ol.2018.7995