Effect of high WDR5 expression on the hepatocellular carcinoma prognosis

Cui,Zhoujun; Li,Hongbo; Liang,Feng; Mu,Cuiling; Mu,Yuhua; Zhang,Xuegong; Liu,Jundong

doi:10.3892/ol.2018.8298

Effect of high WDR5 expression on the hepatocellular carcinoma prognosis

Authors:
- Zhoujun Cui
- Hongbo Li
- Feng Liang
- Cuiling Mu
- Yuhua Mu
- Xuegong Zhang
- Jundong Liu
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Affiliations: Department of General Surgery, People's Hospital of Rizhao, Rizhao, Shandong 276800, P.R. China
Published online on: March 20, 2018 https://doi.org/10.3892/ol.2018.8298
Pages: 7864-7870
Copyright: © Cui et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

WD repeat domain 5 (WDR5) serves an important role in various biological functions through the epigenetic regulation of gene transcription. Aberrant expression of WDR5 has been observed in various types of human cancer, including prostate cancer, breast cancer and leukemia. However, the role of WDR5 expression and its clinical implications in hepatocellular carcinoma (HCC) remain largely unknown. The present study investigated the WDR5 expression pattern in HCC. It was demonstrated that the mRNA and protein levels of WDR5 were upregulated in HCC cancer tissues compared with normal adjacent tissues using reverse transcription‑quantitative polymerase chain reaction and western blotting. Furthermore, the elevated WDR5 protein level was significantly associated with the histological grade (P=0.038), tumor size (P=0.023), tumor‑node‑metastasis stage (P=0.035) and reduced long‑term survival time. Additionally, it was demonstrated through the shRNA‑mediated knockdown of WDR5 in HCC cells in vitro that WDR5 expression promotes cell proliferation using an MTT assay. Taken together, the results suggested that WDR5 overexpression may have an oncogenic effect in HCC, and may be a promising biomarker for the diagnosis and prognosis of HCC.

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