Effect of cytokine‑induced killer cells combined with dendritic cells on the survival rate and expression of 14‑3‑3ζ and p‑Bad proteins in Lewis lung cancer cell lines

  • Authors:
    • Yang Hou
    • Dongyu Zang
    • Xiaoming Li
    • Fuzhi Li
  • View Affiliations

  • Published online on: May 30, 2018     https://doi.org/10.3892/ol.2018.8834
  • Pages: 1815-1820
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

In the present study, the function and mechanism of cytokine‑induced killer cells (CIK) combined with dendritic cells (DC‑CIK) were examined in Lewis lung cancer (LLC) cells. Co‑culture of CIK dendritic cells (DC) in vitro was used to investigate their proliferation and the antitumor effects on LLC cells. DC and CIK cells were collected from healthy human peripheral blood mononuclear cells and co‑cultured as an experimental group, while LLC cells were cultured alone as a control group. Cell morphology was observed by an inverted microscope and an MTT assay was utilized to detect the proliferation of LLC cells. Expression of 14‑3‑3ζ and p‑Bad were measured by western blot analysis. Compared with the control group, treatment of LLC cells with DC‑CIK resulted in decreased cell adherence, reduced cell proliferation and abnormal morphological changes. Additionally, DC‑CIK treatment of LLC cells resulted in the decreased expression of 14‑3‑3ζ and p‑Bad protein in LLC cells, which may provide important information pertaining to the possible mechanism of DC‑CIK‑induced antitumor activity against LLC cells. The present study provides a theoretical and experimental basis for the clinical treatment of DC‑CIK cell co‑culture.
View Figures
View References

Related Articles

Journal Cover

August-2018
Volume 16 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Hou Y, Zang D, Li X and Li F: Effect of cytokine‑induced killer cells combined with dendritic cells on the survival rate and expression of 14‑3‑3ζ and p‑Bad proteins in Lewis lung cancer cell lines. Oncol Lett 16: 1815-1820, 2018.
APA
Hou, Y., Zang, D., Li, X., & Li, F. (2018). Effect of cytokine‑induced killer cells combined with dendritic cells on the survival rate and expression of 14‑3‑3ζ and p‑Bad proteins in Lewis lung cancer cell lines. Oncology Letters, 16, 1815-1820. https://doi.org/10.3892/ol.2018.8834
MLA
Hou, Y., Zang, D., Li, X., Li, F."Effect of cytokine‑induced killer cells combined with dendritic cells on the survival rate and expression of 14‑3‑3ζ and p‑Bad proteins in Lewis lung cancer cell lines". Oncology Letters 16.2 (2018): 1815-1820.
Chicago
Hou, Y., Zang, D., Li, X., Li, F."Effect of cytokine‑induced killer cells combined with dendritic cells on the survival rate and expression of 14‑3‑3ζ and p‑Bad proteins in Lewis lung cancer cell lines". Oncology Letters 16, no. 2 (2018): 1815-1820. https://doi.org/10.3892/ol.2018.8834