Fusobacterium nucleatum promotes the progression of colorectal cancer by interacting with E‑cadherin

  • Authors:
    • Chun‑Ting Ma
    • He‑Sheng Luo
    • Feng Gao
    • Qin‑Cai Tang
    • Wei Chen
  • View Affiliations

  • Published online on: June 11, 2018     https://doi.org/10.3892/ol.2018.8947
  • Pages: 2606-2612
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Increasing evidence suggests that Fusobacterium nucleatum is involved in colorectal carcinogenesis. Previous studies have explored whether F. nucleatum may trigger colonic epithelial‑mesenchymal transition. The results of the present study demonstrated that F. nucleatum enhances the proliferation and invasion of NCM460 cells compared with that of normal control and DH5α cells. Furthermore, F. nucleatum significantly increased the phosphorylation of p65 (a subunit of nuclear factor‑κB), as well as the expression of interleukin (IL)‑6, IL‑1β and matrix metalloproteinase (MMP)‑13. Additionally, F. nucleatum infection did not affect the expression levels of epithelial (E‑)cadherin and β‑catenin. E‑cadherin knockdown in NCM460 cells did not induce the activation of inflammatory responses in response to F. nucleatum infection, whereas it increased inflammation in response to β‑catenin silencing. F. nucleatum infection could not increase the proportion of cells at S phase when E‑cadherin was silenced. Nevertheless, F. nucleatum infection enhanced the proportion of NCM460 cells at S phase when transfected with small interfering RNAs to knock down β‑catenin expression. In conclusion, the results of the present study demonstrated that F. nucleatum infection interacted with E‑cadherin instead of β‑catenin, which in turn enhances the malignant phenotype of colorectal cancer cells.
View Figures
View References

Related Articles

Journal Cover

August-2018
Volume 16 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Ma CT, Luo HS, Gao F, Tang QC and Chen W: Fusobacterium nucleatum promotes the progression of colorectal cancer by interacting with E‑cadherin. Oncol Lett 16: 2606-2612, 2018.
APA
Ma, C., Luo, H., Gao, F., Tang, Q., & Chen, W. (2018). Fusobacterium nucleatum promotes the progression of colorectal cancer by interacting with E‑cadherin. Oncology Letters, 16, 2606-2612. https://doi.org/10.3892/ol.2018.8947
MLA
Ma, C., Luo, H., Gao, F., Tang, Q., Chen, W."Fusobacterium nucleatum promotes the progression of colorectal cancer by interacting with E‑cadherin". Oncology Letters 16.2 (2018): 2606-2612.
Chicago
Ma, C., Luo, H., Gao, F., Tang, Q., Chen, W."Fusobacterium nucleatum promotes the progression of colorectal cancer by interacting with E‑cadherin". Oncology Letters 16, no. 2 (2018): 2606-2612. https://doi.org/10.3892/ol.2018.8947