Open Access

The knockdown of the Mediator complex subunit MED15 restrains urothelial bladder cancer cells' malignancy

  • Authors:
    • Isabella Syring
    • Richard Weiten
    • Tim Müller
    • Doris Schmidt
    • Susanne Steiner
    • Glen Kristiansen
    • Stefan C. Müller
    • Jörg Ellinger
  • View Affiliations

  • Published online on: June 25, 2018     https://doi.org/10.3892/ol.2018.9014
  • Pages: 3013-3021
  • Copyright: © Syring et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The Mediator complex, a multi‑subunit protein complex, plays an integral role in regulating transcription. Genetic alterations of the mediator subunit 15 (MED15) in separate tumor entities have been described previously. However, till now, not much is known about the role of MED15 in urothelial bladder cancer (BCa). Using cBioPortal, database analysis was executed for the mRNA expression and survival analysis of MED15 in BCa. Immunohistochemistry (IHC) analysis against MED15 was performed on tissue microarrays with 18 benign, 126 BCa, and 38 metastases samples. The intensity evaluation was performed using a staining intensity score from 0 to 3 and associated with clinicopathological data. The BCa cell lines T24 and TCCSUP were used for the functional investigation. After the MED15 knockdown by small interfering (si)RNA, cell proliferation, migration and invasion were investigated. On the mRNA level, only a low number of alterations (2%) was found for MED15 in BCa. Due to the small count of events, there were no significant differences or tendencies in survival. For IHC, MED15 was found to have a higher expression in non‑muscle invasive BCa compared with benign and muscle invasive BCa. For survival analysis, no significant differences between samples with or without overexpression of MED15 were found. In the functional analysis, proliferation, migration, and invasion were significantly reduced in BCa‑cells following the transient siRNA‑mediated MED15 knockdown. In summary, MED15 appears to play a role in the tumor parameters proliferation, migration, and invasion in BCa, but further investigations are necessary.
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September-2018
Volume 16 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Syring I, Weiten R, Müller T, Schmidt D, Steiner S, Kristiansen G, Müller SC and Ellinger J: The knockdown of the Mediator complex subunit MED15 restrains urothelial bladder cancer cells' malignancy. Oncol Lett 16: 3013-3021, 2018
APA
Syring, I., Weiten, R., Müller, T., Schmidt, D., Steiner, S., Kristiansen, G. ... Ellinger, J. (2018). The knockdown of the Mediator complex subunit MED15 restrains urothelial bladder cancer cells' malignancy. Oncology Letters, 16, 3013-3021. https://doi.org/10.3892/ol.2018.9014
MLA
Syring, I., Weiten, R., Müller, T., Schmidt, D., Steiner, S., Kristiansen, G., Müller, S. C., Ellinger, J."The knockdown of the Mediator complex subunit MED15 restrains urothelial bladder cancer cells' malignancy". Oncology Letters 16.3 (2018): 3013-3021.
Chicago
Syring, I., Weiten, R., Müller, T., Schmidt, D., Steiner, S., Kristiansen, G., Müller, S. C., Ellinger, J."The knockdown of the Mediator complex subunit MED15 restrains urothelial bladder cancer cells' malignancy". Oncology Letters 16, no. 3 (2018): 3013-3021. https://doi.org/10.3892/ol.2018.9014