Tanshinol upregulates the expression of aquaporin 5 in lung tissue of rats with sepsis

Xu,Jianfeng; Yang,Lei; Dong,Liang

doi:10.3892/ol.2018.9026

Tanshinol upregulates the expression of aquaporin 5 in lung tissue of rats with sepsis

Authors:
- Jianfeng Xu
- Lei Yang
- Liang Dong
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Affiliations: Department of Respiratory Medicine, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China
Published online on: June 26, 2018 https://doi.org/10.3892/ol.2018.9026
Pages: 3290-3296

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Abstract

Aquaporin 5 (AQP‑5) is a water channel protein that is closely associated with non‑small cell lung cancer tissues. The present study aimed to investigate the mechanism of tanshinol treatment on AQP‑5 in the lung tissue of rats with sepsis. Animals in a rat sepsis model were randomly divided into six groups including blank control (ctrl), sham operation (SO), model (sepsis), low dose tanshinol (5 mg/kg/day; Tan‑L), moderate dose tanshinol (10 mg/kg/day; Tan‑M) and high dose tanshinol (20 mg/kg/day; Tan‑H) groups. After 7 days of administration, the expression level of AQP‑5 mRNA was detected by reverse transcription‑quantitative polymerase chain reaction. The levels of interleukin‑6 (IL‑6) and tumor necrosis factor α (TNF‑α) were measured by ELISA. Hematoxylin and eosin staining was used for histopathological observation. The expression levels of AQP‑5, P38 and phosphorylated (P)‑P38 protein in lung tissues were detected by western blot analysis. The expression levels of AQP‑5 in the sepsis group were significantly decreased compared with those in ctrl and SO groups (P<0.01), while the levels of TNF‑α, IL‑6 and p‑P38 were significantly increased in sepsis group compared with those in ctrl and SO groups (P<0.01). Following tanshinol intervention, the expression levels of AQP‑5 were significantly increased, while the levels of TNF‑α, IL‑6 and p‑P38 were decreased compared with those in sepsis group. Tanshinol may upregulate the expression of AQP‑5 by inhibiting the inflammatory cytokines and phosphorylation of P38, therefore protecting the lung tissue of rats with sepsis.

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1	Martin GS: Sepsis, severe sepsis and septic shock: Changes in incidence, pathogens and outcomes. Expert Rev Anti Infect Ther. 10:701–706. 2012. View Article : Google Scholar : PubMed/NCBI
2	Angus DC and van der Poll T: Severe sepsis and septic shock. N Engl J Med. 369:840–851. 2013. View Article : Google Scholar : PubMed/NCBI
3	Dellinger RP, Levy MM, Rhodes A, Annane D, Gerlach H, Opal SM, Sevransky JE, Sprung CL, Douglas IS, Jaeschke R, et al: Surviving sepsis campaign: International guidelines for management of severe sepsis and septic shock, 2012. Intens Care Med. 39:165–228. 2013. View Article : Google Scholar
4	Levy MM, Fink MP, Marshall JC, Abraham E, Angus D, Cook D, Cohen J, Opal SM, Vincent JL and Ramsay G: SCCM/ESICM/ACCP/ATS/SIS: 2001 SCCM/ESICM/ACCP/ATS/SIS International Sepsis Definitions onference. Crit Care Med. 31:1250–1256. 2003. View Article : Google Scholar : PubMed/NCBI
5	Kaukonen KM, Bailey M, Suzuki S, Pilcher D and Bellomo R: Mortality related to severe sepsis and septic shock among critically ill patients in Australia and New Zealand, 2000–2012. JAMA. 311:1308–1316. 2014. View Article : Google Scholar : PubMed/NCBI
6	Bernard GR, Vincent JL, Laterre PF, LaRosa SP, Dhainaut JF, Lopez-Rodriguez A, Steingrub JS, Garber GE, Helterbrand JD, Ely EW, et al: Efficacy and safety of recombinant human activated protein C for severe sepsis. N Engl J Med. 344:699–709. 2001. View Article : Google Scholar : PubMed/NCBI
7	Rivers E, Nguyen B, Havstad S, Ressler J, Muzzin A, Knoblich B, Peterson E and Tomlanovich M: Early Goal-Directed Therapy Collaborative Group: Early goal-directed therapy in the treatment of severe sepsis and septic shock. N Engl J Med. 345:1368–1377. 2001. View Article : Google Scholar : PubMed/NCBI
8	Peake SL, Bailey M, Bellomo R, Cameron PA, Cross A, Delaney A, Finfer S, Higgins A, Jones DA, Myburgh JA, et al: Australasian resuscitation of sepsis evaluation (ARISE): A multi-centre, prospective, inception cohort study. Resuscitation. 80:811–818. 2009. View Article : Google Scholar : PubMed/NCBI
9	Wawrzeniak IC, Loss SH, Moraes MC, De La Vega FL and Victorino JA: Could a protocol based on early goal-directed therapy improve outcomes in patients with severe sepsis and septic shock in the intensive care unit setting? Indian J Crit Care Med. 19:159–165. 2015. View Article : Google Scholar : PubMed/NCBI
10	Zhang Y, Li X and Wang Z: Antioxidant activities of leaf extract of Salvia miltiorrhiza Bunge and related phenolic constituents. Food Chem Toxicol. 48:2656–2662. 2010. View Article : Google Scholar : PubMed/NCBI
11	Son B, Jun SY, Seo H, Youn H, Yang HJ, Kim W, Kim HK, Kang C and Youn B: Inhibitory effect of traditional oriental medicine-derived monoamine oxidase B inhibitor on radioresistance of non-small cell lung cancer. Sci Rep. 6:219862016. View Article : Google Scholar : PubMed/NCBI
12	Guan Y, Wu XX, Duan JL, Yin Y, Guo C, Wei G, Wang YH, Zhu YR, Weng Y, Xi MM and Wen AD: Effects and mechanism of combination of rhein and danshensu in the treatment of chronic kidney disease. Am J Chin Med. 43:1381–1400. 2015. View Article : Google Scholar : PubMed/NCBI
13	Yu J, Wang L, Akinyi M, Li Y, Duan Z, Zhu Y and Fan G: Danshensu protects isolated heart against ischemia reperfusion injury through activation of Akt/ERK1/2/Nrf2 signaling. Int J Clin Exp Med. 8:14793–14804. 2015.PubMed/NCBI
14	Yang Y, Su Y, Wang D, Chen Y, Wu T, Li G, Sun X and Cui L: Tanshinol attenuates the deleterious effects of oxidative stress on osteoblastic differentiation via Wnt/FoxO3a signaling. Oxid Med Cell Longev. 2013:3518952013. View Article : Google Scholar : PubMed/NCBI
15	Liu XH, Pan LL, Jia YL, Wu D, Xiong QH, Wang Y and Zhu YZ: A novel compound DSC suppresses lipopolysaccharide-induced inflammatory responses by inhibition of Akt/NF-κB signalling in macrophages. Eur J Pharmacol. 708:8–13. 2013. View Article : Google Scholar : PubMed/NCBI
16	Yang Y, Wang L, Wu Y, Su D, Wang N, Wang J, Shi C, Lv L and Zhang S: Tanshinol suppresses inflammatory factors in a rat model of vascular dementia and protects LPS-treated neurons via the MST1-FOXO3 signaling pathway. Brain Res. 1646:304–314. 2016. View Article : Google Scholar : PubMed/NCBI
17	Shioji I: Oxidative stress related diseases and biopyrrins. Rinsho Byori. 53:155–159. 2005.(In Japanese). PubMed/NCBI
18	Kubota M, Hasegawa T, Nakakura T, Tanii H, Suzuki M and Tanaka S: Molecular and cellular characterization of a new aquaporin, AQP-x5, specifically expressed in the small granular glands of Xenopus skin. J Exp Biol. 209:3199–3208. 2006. View Article : Google Scholar : PubMed/NCBI
19	Delporte C, Bryla A and Perret J: Aquaporins in salivary glands: From basic research to clinical applications. Int J Mol Sci. 17:pii: E166. 2016. View Article : Google Scholar
20	Verkman AS, Anderson MO and Papadopoulos MC: Aquaporins: Important but elusive drug targets. Nat Rev Drug Discov. 13:259–277. 2014. View Article : Google Scholar : PubMed/NCBI
21	Shen Y, Wang Y, Chen Z, Wang D, Wang X, Jin M and Bai C: Role of aquaporin 5 in antigen-induced airway inflammation and mucous hyperproduction in mice. J Cell Mol Med. 15:1355–1363. 2011. View Article : Google Scholar : PubMed/NCBI
22	Gresz V, Horvath A, Gera I, Nielsen S and Zelles T: Immunolocalization of AQP5 in resting and stimulated normal labial glands and in Sjögren's syndrome. Oral Dis. 21:e114–e120. 2015. View Article : Google Scholar : PubMed/NCBI
23	Lin F, Pan LH, Ruan L, Qian W, Liang R, Ge WY and Huang B: Differential expression of HIF-1α, AQP-1, and VEGF under acute hypoxic conditions in the non-ventilated lung of a one-lung ventilation rat model. Life Sci. 124:50–55. 2015. View Article : Google Scholar : PubMed/NCBI
24	Han G, Ma L, Guo Y, Li L, Li D and Liu H: Hyperbaric oxygen therapy palliates lipopolysaccharide-induced acute lung injury in rats by upregulating AQP1 and AQP5 expression. Exp Lung Res. 41:444–449. 2015. View Article : Google Scholar : PubMed/NCBI
25	Zhang ZQ, Song YL, Chen ZH, Shen Y and Bai CX: Deletion of aquaporin 5 aggravates acute lung injury induced by Pseudomonas aeruginosa. J Trauma. 71:1305–1311. 2011. View Article : Google Scholar : PubMed/NCBI
26	Direito I, Madeira A, Brito MA and Soveral G: Aquaporin-5: From structure to function and dysfunction in cancer. Cell Mol Life Sci. 73:1623–1640. 2016. View Article : Google Scholar : PubMed/NCBI
27	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
28	Govindan S, Iwashyna TJ, Odden A, Flanders SA and Chopra V: Mobilization in severe sepsis: An integrative review. J Hosp Med. 10:54–59. 2015. View Article : Google Scholar : PubMed/NCBI
29	Chen H, Xie K, Han H, Li Y, Liu L, Yang T and Yu Y: Molecular hydrogen protects mice against polymicrobial sepsis by ameliorating endothelial dysfunction via an Nrf2/HO-1 signaling pathway. Int Immunopharmacol. 28:643–654. 2015. View Article : Google Scholar : PubMed/NCBI
30	Yudkin JS, Kumari M, Humphries SE and Mohamed-Ali V: Inflammation, obesity, stress and coronary heart disease: Is interleukin-6 the link? Atherosclerosis. 148:209–214. 2000. View Article : Google Scholar : PubMed/NCBI
31	Khatri M and Sharma JM: Infectious bursal disease virus infection induces macrophage activation via p38 MAPK and NF-kappaB pathways. Virus Res. 118:70–77. 2006. View Article : Google Scholar : PubMed/NCBI
32	Rydkina E, Silverman DJ and Sahni SK: Activation of p38 stress-activated protein kinase during Rickettsia rickettsii infection of human endothelial cells: Role in the induction of chemokine response. Cell Microbiol. 7:1519–1530. 2005. View Article : Google Scholar : PubMed/NCBI