Open Access

Homoisoflavanone‑1 isolated from Polygonatum odoratum arrests the cell cycle and induces apoptosis in A549 cells

  • Authors:
    • Deli Ning
    • Ming Jin
    • Tao Xv
    • Jikai Sun
    • Min Li
  • View Affiliations

  • Published online on: July 5, 2018     https://doi.org/10.3892/ol.2018.9085
  • Pages: 3545-3554
  • Copyright: © Ning et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Homoisoflavanone‑1 is a natural compound that may be extracted from the Chinese medicinal herb Polygonatum odoratum, which has pronounced antioxidant activities. The present study reports that homoisoflavanone‑1 significantly inhibited tumor cell growth and induced apoptosis in A549 non‑small cell lung cancer (NSCLC) cells in a dose‑dependent manner. Homoisoflavanone‑1 arrested the cell cycle at the G2/M stage, which was associated with an increase in the accumulation of phosphorylated (p‑)p38, p38, p53, and p‑cyclin dependent kinase (Cdc)2 proteins, as well as a decrease in Cdc2 expression. In addition, treatment with homoisoflavanone‑1 increased the levels of active caspase‑3 and decreased Poly ADP‑ribose polymerase, which was accompanied by a reduction in the B‑cell lymphoma‑2/Bak ratio and consequently, apoptosis. Furthermore, homoisoflavanone‑1 increased the expression of endoplasmic reticulum (ER) stress‑related proteins, including PERK, ATF4 and GADD34 in a dose‑dependent manner. In conclusion, homoisoflavanone‑1 induced apoptosis in A549 cells by regulating the mitochondria‑caspase‑dependent and ER stress pathways and resulted in G2/M arrest by activating the p38/p53 signaling pathway. These findings suggest that homoisoflavanone‑1 extracted from Polygonatum odoratum may function as a cancer‑suppressing agent and has potential as a novel therapeutic method against NSCLC.
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September-2018
Volume 16 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Ning D, Jin M, Xv T, Sun J and Li M: Homoisoflavanone‑1 isolated from Polygonatum odoratum arrests the cell cycle and induces apoptosis in A549 cells. Oncol Lett 16: 3545-3554, 2018.
APA
Ning, D., Jin, M., Xv, T., Sun, J., & Li, M. (2018). Homoisoflavanone‑1 isolated from Polygonatum odoratum arrests the cell cycle and induces apoptosis in A549 cells. Oncology Letters, 16, 3545-3554. https://doi.org/10.3892/ol.2018.9085
MLA
Ning, D., Jin, M., Xv, T., Sun, J., Li, M."Homoisoflavanone‑1 isolated from Polygonatum odoratum arrests the cell cycle and induces apoptosis in A549 cells". Oncology Letters 16.3 (2018): 3545-3554.
Chicago
Ning, D., Jin, M., Xv, T., Sun, J., Li, M."Homoisoflavanone‑1 isolated from Polygonatum odoratum arrests the cell cycle and induces apoptosis in A549 cells". Oncology Letters 16, no. 3 (2018): 3545-3554. https://doi.org/10.3892/ol.2018.9085