LncRNA ATB promotes the proliferation and metastasis of lung cancer via activation of the p38 signaling pathway

Wei,Lei; Wu,Tao; He,Ping; Zhang,Jun‑Lei; Wu,Wei

doi:10.3892/ol.2018.9117

LncRNA ATB promotes the proliferation and metastasis of lung cancer via activation of the p38 signaling pathway

Authors:
- Lei Wei
- Tao Wu
- Ping He
- Jun‑Lei Zhang
- Wei Wu
View Affiliations

Affiliations: Department of Cardiothoracic Surgery, Southwest Hospital, Chongqing 400038, P.R. China, Stem Cell and Developmental Biology Laboratory, Department of Histology and Embryology, Third Military Medical University, Chongqing 400038, P.R. China
Published online on: July 10, 2018 https://doi.org/10.3892/ol.2018.9117
Pages: 3907-3912

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Long non‑coding RNA (lncRNA) activated by TGF‑β (ATB) has been reported to be widely expressed in different types of cancer; however, the function of ATB in lung cancer remains unclear. In order to elucidate the role of ATB in lung cancer, reverse transcription‑quantitative polymerase chain reaction was used to detect the expression of ATB in tumor tissues and corresponding non‑tumor lung tissues from 32 patients with lung cancer. Furthermore, the association between the expression of ATB and clinical characteristics was investigated. Cell proliferation was assessed using a cell counting kit‑8 assay and cell migration was assessed using a wound healing assays. Epithelial‑mesenchymal‑transition and mitogen‑activated protein kinase signaling pathway activity was examined using western blotting. It was demonstrated that ATB was highly expressed in lung cancer tissues compared with noncancerous tissues, and associated with tumor size and metastasis. It was also demonstrated that ATB was highly expressed in the lung cancer cell lines, A549 and HCC827, compared with the HBE‑1 cell line. Suppression of ATB significantly inhibited the proliferation and migratory rate of lung cancer cells. The protein expression levels of p38, E‑cadherin and N‑cadherin were altered by suppression of ATB expression. Overall, the present study demonstrated that ATB may promote the development of lung cancer.

View Figures

View References

1	Hao Y, Yang X, Zhang D, Luo J and Chen R: Long noncoding RNA LINC01186, regulated by TGF-β/SMAD3, inhibits migration and invasion through Epithelial-Mesenchymal-Transition in lung cancer. Gene. 608:1–12. 2017. View Article : Google Scholar : PubMed/NCBI
2	Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI
3	Sang H, Liu H, Xiong P and Zhu M: Long non-coding RNA functions in lung cancer. Tumour Biol. 36:4027–4037. 2015. View Article : Google Scholar : PubMed/NCBI
4	Liu T, Chi H, Chen J, Chen C, Huang Y, Xi H, Xue J and Si Y: Curcumin suppresses proliferation and in vitro invasion of human prostate cancer stem cells by ceRNA effect of miR-145 and lncRNA-ROR. Gene. 631:29–38. 2017. View Article : Google Scholar : PubMed/NCBI
5	Wang ZQ, He CY, Hu L, Shi HP, Li JF, Gu QL, Su LP, Liu BY, Li C and Zhu Z: Long noncoding RNA UCA1 promotes tumour metastasis by inducing GRK2 degradation in gastric cancer. Cancer Lett. 408:10–21. 2017. View Article : Google Scholar : PubMed/NCBI
6	Chen DL, Chen LZ, Lu YX, Zhang DS, Zeng ZL, Pan ZZ, Huang P, Wang FH, Li YH, Ju HQ and Xu RH: Long noncoding RNA XIST expedites metastasis and modulates epithelial-mesenchymal transition in colorectal cancer. Cell Death Dis. 8:e30112017. View Article : Google Scholar : PubMed/NCBI
7	Wang H, Huo X, Yang XR, He J, Cheng L, Wang N, Deng X, Jin H, Wang N, Wang C, et al: STAT3-mediated upregulation of lncRNA HOXD-AS1 as a ceRNA facilitates liver cancer metastasis by regulating SOX4. Mol Cancer. 16:1362017. View Article : Google Scholar : PubMed/NCBI
8	Zhao Z, Wang J, Wang S, Chang H, Zhang T and Qu J: LncRNA CCAT2 promotes tumorigenesis by over-expressed Pokemon in non-small cell lung cancer. Biomed Pharmacother. 87:692–697. 2017. View Article : Google Scholar : PubMed/NCBI
9	Roth A and Diederichs S: Long noncoding RNAs in lung cancer. Curr Top Microbiol Immunol. 394:57–110. 2016.PubMed/NCBI
10	Yuan JH, Yang F, Wang F, Ma JZ, Guo YJ, Tao QF, Liu F, Pan W, Wang TT, Zhou CC, et al: A long noncoding RNA activated by TGF-β promotes the invasion-metastasis cascade in hepatocellular carcinoma. Cancer Cell. 25:666–681. 2014. View Article : Google Scholar : PubMed/NCBI
11	Shi SJ, Wang LJ, Yu B, Li YH, Jin Y and Bai XZ: LncRNA-ATB promotes trastuzumab resistance and invasion-metastasis cascade in breast cancer. Oncotarget. 6:11652–63. 2015. View Article : Google Scholar : PubMed/NCBI
12	Ma CC, Xiong Z, Zhu GN, Wang C, Zong G, Wang HL, Bian EB and Zhao B: Long non-coding RNA ATB promotes glioma malignancy by negatively regulating miR-200a. J Exp Clin Cancer Res. 35:902016. View Article : Google Scholar : PubMed/NCBI
13	Yue B, Qiu S, Zhao S, Liu C, Zhang D, Yu F, Peng Z and Yan D: LncRNA-ATB mediated E-cadherin repression promotes the progression of colon cancer and predicts poor prognosis. J Gastroenterol Hepatol. 31:595–603. 2016. View Article : Google Scholar : PubMed/NCBI
14	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
15	Khan GJ, Gao Y, Gu M, Wang L, Khan S, Naeem F, Yousef BA, Roy D, Semukunzi H, Yuan S and Sun L: TGF-β1 causes EMT by regulating N-Acetyl Glucosaminyl transferases via downregulation of non muscle Myosin II-A through JNK/P38/PI3K pathway in lung cancer. Curr Cancer Drug Targets. 18:209–219. 2018. View Article : Google Scholar : PubMed/NCBI
16	Ke L, Xu SB, Wang J, Jiang XL and Xu MQ: High expression of long non-coding RNA ATB indicates a poor prognosis and regulates cell proliferation and metastasis in non-small cell lung cancer. Clin Transl Oncol. 19:599–605. 2017. View Article : Google Scholar : PubMed/NCBI
17	van der Aalst CM, Ten Haaf K and de Koning HJ: Lung cancer screening: Latest developments and unanswered questions. Lancet Respir Med. 4:749–761. 2016. View Article : Google Scholar : PubMed/NCBI
18	Checa M, Hagood JS, Velazquez-Cruz R, Ruiz V, Garcia-De-Alba C, Rangel-Escareno C, Urrea F, Becerril C, Montano M, Garcia-Trejo S, et al: Cigarette smoke enhances the expression of profibrotic molecules in alveolar epithelial cells. PLoS One. 11:e01503832016. View Article : Google Scholar : PubMed/NCBI
19	Islas-Vazquez L, Prado-Garcia H, Aguilar-Cazares D, Meneses-Flores M, Galicia-Velasco M, Romero-Garcia S, Camacho-Mendoza C and Lopez-Gonzalez JS: LAP TGF-Beta subset of CD4(+)CD25(+)CD127(−) Treg cells is increased and overexpresses LAP TGF-Beta in lung adenocarcinoma patients. Biomed Res Int. 2015:4309432015. View Article : Google Scholar : PubMed/NCBI
20	Han F, Wang C, Wang Y and Zhang L: Long noncoding RNA ATB promotes osteosarcoma cell proliferation, migration and invasion by suppressing miR-200s. Am J Cancer Res. 7:770–783. 2017.PubMed/NCBI
21	Xiao Y, Yan W, Lu L, Wang Y, Lu W, Cao Y and Cai W: p38/p53/miR-200a-3p feedback loop promotes oxidative stress-mediated liver cell death. Cell Cycle. 14:1548–1558. 2015. View Article : Google Scholar : PubMed/NCBI
22	Chen T, Gong W, Tian H, Wang H, Chu S, Ma J, Yang H, Cheng J, Liu M, Li X and Jiang C: Fibroblast growth factor 18 promotes proliferation and migration of H460 cells via the ERK and p38 signaling pathways. Oncol Rep. 37:1235–1242. 2017. View Article : Google Scholar : PubMed/NCBI