Silencing of uPAR via RNA interference inhibits invasion and migration of oral tongue squamous cell carcinoma

  • Authors:
    • Xuexiang Gao
    • Qi Guo
    • Shuo Wang
    • Cen Gao
    • Jian Chen
    • Li Zhang
    • Yuan Zhao
    • Jing Wang
  • View Affiliations

  • Published online on: July 6, 2018     https://doi.org/10.3892/ol.2018.9094
  • Pages: 3983-3991
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Abstract

Overexpression of urokinase‑type plasminogen activator receptor (uPAR) has been implicated in promoting tumor invasion in various cancer types, including oral tongue squamous cell carcinoma (OTSCC); therefore, the effect of suppressing uPAR expression on the invasive and metastatic potential of OTSCC was investigated. A total of 65 paraffin‑embedded tissues were obtained from patients with OTSCC. Immunohistochemistry was used to determine the expression level of uPAR. The Ts cells transfected with short hairpin RNA targeting uPAR were constructed and validated. The cells were used in a number of in vitro analyses, including migration, invasion and western blot analysis assays. Furthermore, a mouse lung metastatic model was used to detect the metastatic ability of OTSCC cells in the lungs. OTSCC cell metastasis and relapse were determined to be associated with uPAR immunopositivity. Inhibition of uPAR expression in Ts cells demonstrated a 40% decrease in migration and a 60% decrease in invasion in vitro, with an associated downregulation of matrix metalloprotease (MMP)‑2, MMP‑9 and phosphorylated extracellular signal‑regulated kinase. In vivo analysis indicated a 90% decrease in the number of mice bearing macroscopic lung metastases. In conclusion, the present study demonstrated that the targeting of uPAR‑inhibited cellular proliferation and invasion would provide a potential treatment for OTSCC in the future.
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September-2018
Volume 16 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Gao X, Guo Q, Wang S, Gao C, Chen J, Zhang L, Zhao Y and Wang J: Silencing of uPAR via RNA interference inhibits invasion and migration of oral tongue squamous cell carcinoma. Oncol Lett 16: 3983-3991, 2018
APA
Gao, X., Guo, Q., Wang, S., Gao, C., Chen, J., Zhang, L. ... Wang, J. (2018). Silencing of uPAR via RNA interference inhibits invasion and migration of oral tongue squamous cell carcinoma. Oncology Letters, 16, 3983-3991. https://doi.org/10.3892/ol.2018.9094
MLA
Gao, X., Guo, Q., Wang, S., Gao, C., Chen, J., Zhang, L., Zhao, Y., Wang, J."Silencing of uPAR via RNA interference inhibits invasion and migration of oral tongue squamous cell carcinoma". Oncology Letters 16.3 (2018): 3983-3991.
Chicago
Gao, X., Guo, Q., Wang, S., Gao, C., Chen, J., Zhang, L., Zhao, Y., Wang, J."Silencing of uPAR via RNA interference inhibits invasion and migration of oral tongue squamous cell carcinoma". Oncology Letters 16, no. 3 (2018): 3983-3991. https://doi.org/10.3892/ol.2018.9094