Circulating long non‑coding RNAs HULC and ZNFX1‑AS1 are potential biomarkers in patients with gastric cancer

Xian,Hai‑Peng; Zhuo,Zhong‑Ling; Sun,Yu‑Jing; Liang,Bin; Zhao,Xiao‑Tao

doi:10.3892/ol.2018.9199

Circulating long non‑coding RNAs HULC and ZNFX1‑AS1 are potential biomarkers in patients with gastric cancer

Authors:
- Hai‑Peng Xian
- Zhong‑Ling Zhuo
- Yu‑Jing Sun
- Bin Liang
- Xiao‑Tao Zhao
View Affiliations

Affiliations: Department of Clinical Laboratory, Peking University People's Hospital, Beijing 100044, P.R. China, Department of Clinical Laboratory, Peking University International Hospital, Beijing 102206, P.R. China, Department of Gastroenterological Surgery, Peking University People's Hospital, Beijing 100044, P.R. China
Published online on: July 24, 2018 https://doi.org/10.3892/ol.2018.9199
Pages: 4689-4698

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Long non‑coding RNAs (lncRNAs) have been demonstrated to be involved in different types of cancer, including gastric cancer. Although altered lncRNAs profiles have been observed in or around gastric cancer tissues, the diagnostic value of circulating lncRNAs in gastric cancer remains unclear. In the present study, a number of highly expressed lncRNAs, including uc001lsz, GACAT2, ABHD11‑AS1, GACAT3, SUMP1P3, CHET1, TUG1, SNHG12, GAS5, PVT1, LINC00152, HOTAIR, CCAT1, H19, HULC and ZNFX1‑AS1, were investigated as potential minimally invasive biomarkers for this tumor. Preliminary screening experiments revealed that ZNFX1‑AS1 and HULC were differentially expressed in the plasma of gastric cancer patients and healthy control subjects. The study further examined the relative expression of ZNFX1‑AS1 and HULC in the plasma of 50 matching preoperative and postoperative patients, 50 gastrointestinal stromal tumor (GIST) patients, 50 gastritis/peptic ulcer patients and 50 healthy control subjects through reverse transcription‑quantitative polymerase chain reaction. The correlation of lncRNA relative expression with the general characteristics and clinicopathological factors was analyzed. It was observed that the levels of ZNFX1‑AS1 and HULC in the plasma of preoperative patients were markedly higher compared with those in the plasma of GIST patients, gastritis/peptic ulcer patients and healthy control subjects, while no significant difference was detected among these three groups. Receiver operating characteristic curve analysis was also conducted to distinguish gastric cancer patients from healthy control subjects. The area under the curve was 0.85 and 0.65 for ZNFX1‑AS1 and HULC, respectively. In conclusion, the results indicated that the lncRNAs ZNFX1‑AS1 and HULC are promising in the clinical diagnosis of gastric cancer.

View Figures

View References

1	Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI
2	Sun J, Song Y, Chen X, Zhao J, Gao P, Huang X, Xu H and Wang Z: Novel long non-coding RNA RP11-119F7.4 as a potential biomarker for the development and progression of gastric cancer. Oncol Lett. 10:115–120. 2015. View Article : Google Scholar : PubMed/NCBI
3	Hauptman N and Glavač D: Long non-coding RNA in cancer. Int J Mol Sci. 14:4655–4669. 2013. View Article : Google Scholar : PubMed/NCBI
4	Vance KW, Sansom SN, Lee S, Chalei V, Kong L, Cooper SE, Oliver PL and Ponting CP: The long non-coding RNA Paupar regulates the expression of both local and distal genes. EMBO J. 33:296–311. 2014. View Article : Google Scholar : PubMed/NCBI
5	Wapinski O and Chang HY: Long noncoding RNAs and human disease. Trends Cell Biol. 21:354–361. 2011. View Article : Google Scholar : PubMed/NCBI
6	Qi P, Xu MD, Ni SJ, Huang D, Wei P, Tan C, Zhou XY and Du X: Low expression of LOC285194 is associated with poor prognosis in colorectal cancer. J Transl Med. 11:1222013. View Article : Google Scholar : PubMed/NCBI
7	Mei D, Song H, Wang K, Lou Y, Sun W, Liu Z, Ding X and Guo J: Up-regulation of SUMO1 pseudogene 3 (SUMO1P3) in gastric cancer and its clinical association. Med Oncol. 30:7092013. View Article : Google Scholar : PubMed/NCBI
8	Song B, Guan Z, Liu F, Sun D, Wang K and Qu H: Long non-coding RNA HOTAIR promotes HLA-G expression via inhibiting miR-152 in gastric cancer cells. Biochem Biophys Res Commun. 464:807–813. 2015. View Article : Google Scholar : PubMed/NCBI
9	Zhuang M, Gao W, Xu J, Wang P and Shu Y: The long non-coding RNA H19-derived miR-675 modulates human gastric cancer cell proliferation by targeting tumor suppressor RUNX1. Biochem Biophys Res Commun. 448:315–322. 2014. View Article : Google Scholar : PubMed/NCBI
10	Arita T, Ichikawa D, Konishi H, Komatsu S, Shiozaki A, Shoda K, Kawaguchi T, Hirajima S, Nagata H, Kubota T, et al: Circulating long non-coding RNAs in plasma of patients with gastric cancer. Anticancer Res. 33:3185–3193. 2013.PubMed/NCBI
11	Fang XY, Pan HF, Leng RX and Ye DQ: Long noncoding RNAs: Novel insights into gastric cancer. Cancer Lett. 356:357–366. 2015. View Article : Google Scholar : PubMed/NCBI
12	Zhang E, Li W, Yin D, De W, Zhu L, Sun S and Han L: c-Myc-regulated long non-coding RNA H19 indicates a poor prognosis and affects cell proliferation in non-small-cell lung cancer. Tumor Biol. 37:4007–4015. 2016. View Article : Google Scholar
13	Kim HL, Puymon MR, Qin M, et al: NCCN Clinical Practice Guidelines in Oncology. 2013.PubMed/NCBI
14	Bosman FT, Carneiro F, Hruban RH and Theise ND: WHO classification of tumours of the digestive system. Fourth Edition. World Health Organization. 3:4172010.
15	Washington K: 7th edition of the AJCC cancer staging manual: Stomach. Ann Surg Oncol. 17:3077–3079. 2010. View Article : Google Scholar : PubMed/NCBI
16	Xia CS, Fan CH and Yang TS: Establishing reference intervals for serum alpha-fetoprotein and carcinoembryonic antigen in the normal population. Chin J Lab Diagnos. 448:31–35. 2013.(In Chinese).
17	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
18	Resende C, Thiel A, Machado JC and Ristimäki A: Gastric cancer: Basic aspects. Helicobacter. 16 Suppl 1:S38–S44. 2011. View Article : Google Scholar
19	Timms JF, Arslan-Low E, Kabir M, Worthington J, Camuzeaux S, Sinclair J, Szaub J, Afrough B, Podust VN, Fourkala EO, et al: Discovery of serum biomarkers of ovarian cancer using complementary proteomic profiling strategies. Proteomics Clin Appl. 8:982–993. 2014. View Article : Google Scholar : PubMed/NCBI
20	Bagaria B, Sood S, Sharma R and Lalwani S: Comparative study of CEA and CA19-9 in esophageal, gastric and colon cancers individually and in combination (ROC curve analysis). Cancer Biol Med. 10:148–157. 2013.PubMed/NCBI
21	Chen W, Zheng R, Zeng H, Zhang S and He J: Annual report on status of cancer in China, 2011. Chin J Cancer Res. 27:2–12. 2015. View Article : Google Scholar : PubMed/NCBI
22	Arita T, Ichikawa D, Konishi H, Komatsu S, Shiozaki A, Shoda K, Kawaguchi T, Hirajima S, Nagata H, Kubota T, et al: Circulating long non-coding RNAs in plasma of patients with gastric cancer. Anticancer Res. 33:3185–3193. 2013.PubMed/NCBI
23	Liu X, Chen Z, Zhao X, Huang M, Wang C, Peng W, Yin J, Li J, He G, Li X and Zhu X: Effects of IGF2BP2, KCNQ1 and GCKR polymorphisms on clinical outcome in metastatic gastric cancer treated with EOF regimen. Pharmacogenomics. 16:959–970. 2015. View Article : Google Scholar : PubMed/NCBI
24	Shimada H, Noie T, Ohashi M, Oba K and Takahashi Y: Clinical significance of serum tumor markers for gastric cancer: A systematic review of literature by the Task Force of the Japanese Gastric Cancer Association. Gastric Cancer. 17:26–33. 2014. View Article : Google Scholar : PubMed/NCBI
25	Askarian-Amiri ME, Crawford J, French JD, Smart CE, Smith MA, Clark MB, Ru K, Mercer TR, Thompson ER, Lakhani SR, et al: SNORD-host RNA Zfas1 is a regulator of mammary development and a potential marker for breast cancer. RNA. 17:878–891. 2011. View Article : Google Scholar : PubMed/NCBI
26	Zhang JJ, Chen JT, Yao KH, Hua L, Wang CY and Hu JH: Up-regulated expression of long non-coding RNA ZFAS1 associates with aggressive tumor progression and poor prognosis in gastric cancer patients. Int J Clin Exp Pathol. 9:2059–2063. 2016.