MicroRNA‑342‑3p functions as a tumor suppressor by targeting LIM and SH3 protein 1 in oral squamous cell carcinoma

Song,Xiaoyan; Jin,Yong; Yan,Mingyu; Zhang,Yonggang; Chen,Bing

doi:10.3892/ol.2018.9637

MicroRNA‑342‑3p functions as a tumor suppressor by targeting LIM and SH3 protein 1 in oral squamous cell carcinoma

Authors:
- Xiaoyan Song
- Yong Jin
- Mingyu Yan
- Yonggang Zhang
- Bing Chen
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Affiliations: Department of Stomatology, Inner Mongolia Autonomous Region Maternal and Child Health Hospital, Hohhot, Inner Mongolia 010020, P.R. China, Department of Stomatology, Tong‑Liao City Hospital of Inner Mongolia, Tong Liao, Inner Mongolia 028000, P.R. China, The Third Affiliated Hospital, Inner Mongolia Medical University, Baotou, Inner Mongolia 014010, P.R. China, Department of General Surgery, Affiliated Renmin Hospital of Inner Mongolia Medical University, Huhhot, Inner Mongolia 010017, P.R. China, Department of General Surgery, Inner Mongolia Autonomous Region Maternal and Child Health Hospital, Huhhot, Inner Mongolia 010020, P.R. China
Published online on: October 29, 2018 https://doi.org/10.3892/ol.2018.9637
Pages: 688-696

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Abstract

Although microRNA‑342‑3p (miR‑342‑3p) deregulation has been implicated in the development of a variety of cancer types, its role in oral squamous cell carcinoma (OSCC) progression remains unclear. Overexpression of LIM and SH3 protein 1 (LASP1) in OSCC tissues, and its promotion of OSCC cell proliferation were recently reported. However, the regulatory mechanism underlining LASP1 expression remains unknown. In the present study, the notable downregulation of miR‑342‑3p in OSCC cell lines and clinical specimens was revealed. The Cell Counting kit‑8 and 5‑bromo‑2‑deoxyuridine‑incorporation assays demonstrated that miR‑342‑3p suppressed OSCC cell proliferation. Additionally, LASP1 was identified as a target gene of miR‑342‑3p through bioinformatics analysis and luciferase reporter assays. Further experiments suggested that overexpression of LASP1 attenuated the suppressive effect of miR‑342‑3p on the proliferation of OSCC cells. In conclusion, the present data suggest that miR‑342‑3p functions as a tumor suppressor in OSCC via targeting of LASP1 and may be a promising therapeutic target for OSCC.

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