MicroRNA‑525 enhances chondrosarcoma malignancy by targeting F‑spondin 1

Liu,Bo; Song,Xiandong; Yan,Zhaowei; Yang,Hao; Shi,Yingchao; Wu,Jintao

doi:10.3892/ol.2018.9711

MicroRNA‑525 enhances chondrosarcoma malignancy by targeting F‑spondin 1

Authors:
- Bo Liu
- Xiandong Song
- Zhaowei Yan
- Hao Yang
- Yingchao Shi
- Jintao Wu
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Affiliations: Orthopedics Department Two, Hongqi Hospital, Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, P.R. China, Department of Cardiology, Hongqi Hospital, Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, P.R. China, Department of Digestive Disease, Hongqi Hospital, Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, P.R. China
Published online on: November 15, 2018 https://doi.org/10.3892/ol.2018.9711
Pages: 781-788
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Increasing evidence has suggested that microRNAs (miRNAs; miRs) are extensively involved in the progression of chondrosarcoma (CHS). However, few studies have investigated the functional role of miR‑525 in CHS tissues and cells. In the present study, it was discovered that miR‑525 levels were decreased in CHS tissues and cells. Dual luciferase assays indicated that F‑spondin 1 (SPON1) is a target gene of microRNA (miR)‑525. In addition, miR‑525 overexpression suppressed SW1353 cell migration and invasion and enhanced SW1353 cell apoptosis. Increased SPON1 expression levels were identified in CHS tissues and cell lines. Furthermore, miR‑525 overexpression significantly suppressed the activation of focal adhesion kinase (FAK)/Src/phosphatidylinositol‑4,5‑bisphosphate 3‑kinase (PI3K)/protein kinase B (Akt) signaling in CHS cells; this suppression led to SPON1 silencing. In comparison, the SPON1 knockdown‑mediated inactivation of FAK/Src/PI3K/Akt signaling was inhibited by inhibiting miR‑525. In summary, the present study revealed that decreased miR‑525 levels could enhance CHS malignancy as decreased miR‑525 binding to the 3' untranslated region of SPON1 activates FAK/Src/PI3K/Akt signaling.

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