Grape seed proanthocyanidins inhibit proliferation of pancreatic cancer cells by modulating microRNA expression

Wang,Weihua; Zhan,Leilei; Guo,Dongqi; Xiang,Yanju; Tian,Muxing; Zhang,Yu; Wu,Hong; Wei,Yaxun; Ma,Ganglong; Han,Zhanjiang

doi:10.3892/ol.2019.9887

Grape seed proanthocyanidins inhibit proliferation of pancreatic cancer cells by modulating microRNA expression

Authors:
- Weihua Wang
- Leilei Zhan
- Dongqi Guo
- Yanju Xiang
- Muxing Tian
- Yu Zhang
- Hong Wu
- Yaxun Wei
- Ganglong Ma
- Zhanjiang Han
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Affiliations: College of Life Science, Tarim University, Alar, Xinjiang 843300, P.R. China, Center for Genome Analysis, ABLife Inc., Wuhan, Hubei 430075, P.R. China
Published online on: January 4, 2019 https://doi.org/10.3892/ol.2019.9887
Pages: 2777-2787
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs (miRNAs) are small non‑coding RNAs of 18‑25 nucleotides that modulate gene expression at the post‑transcriptional level. Grape seed proanthocyanidins (GSPs), which are biologically active components in grape seeds, have been demonstrated to exhibit anticancer effects. The current study investigated whether GSPs can regulate miRNA expression and the possible anticancer molecular mechanisms of GSPs. Pancreatic cancer (PC) cell samples, SS3, SS12 and SS24, were treated with 20 µg/ml GSPs for 3, 12 and 24 h, respectively. Control samples, SC3, SC12 and SC24, were also prepared. Using miRNA‑seq, transcriptome analysis identified 24, 83 and 83 differentially expressed (DE) miRNAs in SS3 vs. SC3, SS12 vs. SC12 and SS24 vs. SC24, respectively. This indicated that treatment with GSPs could modulate the expression of miRNAs. Subsequently, 74, 598 and 1,204 target genes for the three sets of DE miRNAs were predicted. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis revealed that multiple target genes were associated with the proliferation and apoptosis of PC cells. In addition, a network was constructed of the DE miRNAs and the target genes associated with PC. The associations identified suggested that treatment with GSPs may inhibit the proliferation of PC cells through the modulation of miRNA expression.

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