CD151 confers metastatic potential to clear cell sarcoma of the soft tissue in animal model

Kawashima,Keisuke; Saigo,Chiemi; Kito,Yusuke; Hanamatsu,Yuki; Egawa,Yuki; Takeuchi,Tamotsu

doi:10.3892/ol.2019.10164

CD151 confers metastatic potential to clear cell sarcoma of the soft tissue in animal model

Authors:
- Keisuke Kawashima
- Chiemi Saigo
- Yusuke Kito
- Yuki Hanamatsu
- Yuki Egawa
- Tamotsu Takeuchi
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Affiliations: Department of Pathology and Translational Research, Gifu University Graduate School of Medicine, Gifu 501‑1194, Japan, Division of Pathology, Shizuoka City Shizuoka Hospital, Shizuoka 420‑630, Japan
Published online on: March 19, 2019 https://doi.org/10.3892/ol.2019.10164
Pages: 4811-4818
Copyright: © Kawashima et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cluster of differentiation 151 (CD151) is a potent therapeutic target for regulating tumor metastasis. In the present study, the role of CD151 in clear cell sarcoma of soft tissue was examined using a xenoplanted tumor model, which had high rates of metastasis. A clear cell sarcoma cell line, HS‑MM, which was transplanted to the aponeuroses of the thighs, the most affected sites of human clear cell sarcoma, exhibited robust lymphatic invasion and nodal metastasis in SCID‑beige mice. Serial in vivo passaging of peritoneally disseminated tumor cells accelerated the metastatic activity, which was accompanied by increased CD151 expression, and were designated as HS‑MMhigh. Notably, inoculation of anti‑CD151 antibody significantly suppressed the lymphatic invasion, peritoneal dissemination and distant metastasis of the present clear cell sarcoma model without affecting local tumor growth at the transplantation site. Small interfering RNA (siRNA)‑mediated downregulation of CD151 did not alter cell proliferation, but significantly inhibited Matrigel invasion activity of HS‑MMhigh cells. Downregulation of CD151 impaired matrix metalloproteinase‑9 activity and phosphorylation of SMAD3 protein in HS‑MMhigh cells. The present results suggest that CD151 may contribute to invasion and metastasis of clear cell sarcoma of soft tissue. Therefore, CD151 may serve as a potent target to regulate metastasis of clear cell sarcoma.

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