Effect of lidocaine and ropivacaine on primary (SW480) and metastatic (SW620) colon cancer cell lines

Siekmann,Wiebke; Tina,Elisabet; Koskela von Sydow,Anita; Gupta,Anil

doi:10.3892/ol.2019.10332

Effect of lidocaine and ropivacaine on primary (SW480) and metastatic (SW620) colon cancer cell lines

Authors:
- Wiebke Siekmann
- Elisabet Tina
- Anita Koskela von Sydow
- Anil Gupta
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Affiliations: Department of Anesthesiology and Intensive Care, University Hospital Örebro and School of Medical Sciences, Örebro University, SE‑70185 Örebro, Sweden, Department of Clinical Research Laboratory, Faculty of Medicine and Health, Örebro University, SE‑70185 Örebro, Sweden, Department of Physiology and Pharmacology, Karolinska Institute and Karolinska University Hospital, SE‑17176 Stockholm, Sweden
Published online on: May 7, 2019 https://doi.org/10.3892/ol.2019.10332
Pages: 395-401

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Abstract

Regional anesthesia may prolong survival following surgery for different types of cancers. The mechanisms behind this are unclear but direct effects on cancer cells by local anesthetics (LA) have been suggested. The aim of this study was to investigate if lidocaine or ropivacaine have a dose‑dependent effect on the cell viability and proliferation of a primary and a secondary colon carcinoma cell line in vitro. The colon cancer cell lines SW480 derived from primary tumor and SW620 from a metastatic site in the same patient were exposed to increasing concentrations of lidocaine and ropivacaine (5‑1,000 µM). Cell viability was measured using CellTiter‑Blue® and cell proliferation by PKH67 after exposure for up to 72 h. Cell viability was significantly reduced by ropivacaine at the highest concentration (1,000 µM) after 48 and 72 h in the cell line SW480 and at 72 h in SW620. Exposure to lidocaine did not show any significant reduction in cell viability. Notably, low concentrations of both lidocaine and ropivacaine significantly increased cell viability after 48 and 72 h in SW620. Cell proliferation was significantly reduced by 1,000 µM lidocaine in SW480 and by 1,000 µM ropivacaine in SW620. In summary, both lidocaine and ropivacaine showed an anti‑proliferative effect in the colon cancer cell lines at high concentrations and after prolonged exposure to LA in vitro. Our findings also indicate that lower concentrations promote cell viability in the metastatic cell line.

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