Open Access

MicroRNA‑410‑3p upregulation suppresses proliferation, invasion and migration, and promotes apoptosis in rhabdomyosarcoma cells

  • Authors:
    • Liang Zhang
    • Yuwen Pang
    • Xiaobin Cui
    • Wei Jia
    • Wenwen Cui
    • Yang Liu
    • Chunxia Liu
    • Feng Li
  • View Affiliations

  • Published online on: May 13, 2019     https://doi.org/10.3892/ol.2019.10345
  • Pages: 936-943
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Rhabdomyosarcoma (RMS) is one of the most common types of soft tissue sarcoma in children; however, the pathogenesis of RMS is unclear. MicroRNAs (miRs) are involved in the development and progression of RMS. The role of miR‑410‑3p in RMS cell invasion, migration, proliferation and apoptosis, and its possible mechanism were investigated in the current study. Reverse transcription‑quantitative polymerase chain reaction and western blot analysis were performed to detect the expression of miR‑410‑3p in RMS tissues and cells. In addition, the present study investigated the expression levels of molecules associated with the epithelial‑mesenchymal transition (EMT), including E‑cadherin, N‑cadherin, Slug and Snail, and apoptotic factors, including Bcl‑2‑associated X protein (bax), cleaved‑caspase 3, cleaved poly (ADP‑ribose) polymerase (PARP), p53 and Bcl‑2. Cell Counting Kit‑8, terminal deoxynucleotidyl transferase dUTP nick end labeling and Transwell assays were conducted to determine the functional roles of miR‑410‑3p. Exogenous expression of miR‑410‑3p inhibited RMS cell invasion, migration and proliferation, induced apoptosis, suppressed the expression of Snail, Slug, N‑cadherin and Bcl‑2, and increased the expression of E‑cadherin, bax, cleaved‑caspase 3, cleaved PARP and p53. In summary, it was proposed that miR‑410‑3p overexpression suppressed invasion, migration and proliferation, downregulated the expression of EMT‑associated molecules, and promoted apoptosis and the expression of apoptotic factors in RMS cells. Therefore, miR‑410‑3p may serve as a novel tumor suppressor gene in RMS, and could possess diagnostic and therapeutic potentials for the treatment of RMS.
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July-2019
Volume 18 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhang L, Pang Y, Cui X, Jia W, Cui W, Liu Y, Liu C and Li F: MicroRNA‑410‑3p upregulation suppresses proliferation, invasion and migration, and promotes apoptosis in rhabdomyosarcoma cells. Oncol Lett 18: 936-943, 2019
APA
Zhang, L., Pang, Y., Cui, X., Jia, W., Cui, W., Liu, Y. ... Li, F. (2019). MicroRNA‑410‑3p upregulation suppresses proliferation, invasion and migration, and promotes apoptosis in rhabdomyosarcoma cells. Oncology Letters, 18, 936-943. https://doi.org/10.3892/ol.2019.10345
MLA
Zhang, L., Pang, Y., Cui, X., Jia, W., Cui, W., Liu, Y., Liu, C., Li, F."MicroRNA‑410‑3p upregulation suppresses proliferation, invasion and migration, and promotes apoptosis in rhabdomyosarcoma cells". Oncology Letters 18.1 (2019): 936-943.
Chicago
Zhang, L., Pang, Y., Cui, X., Jia, W., Cui, W., Liu, Y., Liu, C., Li, F."MicroRNA‑410‑3p upregulation suppresses proliferation, invasion and migration, and promotes apoptosis in rhabdomyosarcoma cells". Oncology Letters 18, no. 1 (2019): 936-943. https://doi.org/10.3892/ol.2019.10345