Arsenic compounds induce apoptosis through caspase pathway activation in MA‑10 Leydig tumor cells

Mu,Yi‑Fen; Chen,Ying‑Hui; Chang,Ming‑Min; Chen,Yung‑Chia; Huang,Bu‑Miin

doi:10.3892/ol.2019.10386

Arsenic compounds induce apoptosis through caspase pathway activation in MA‑10 Leydig tumor cells

Authors:
- Yi‑Fen Mu
- Ying‑Hui Chen
- Ming‑Min Chang
- Yung‑Chia Chen
- Bu‑Miin Huang
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Affiliations: Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan, R.O.C., Department of Anesthesia, Chi‑Mei Medical Center, Liouying, Tainan 73657, Taiwan, R.O.C., Department of Anatomy, School of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan, R.O.C.
Published online on: May 21, 2019 https://doi.org/10.3892/ol.2019.10386
Pages: 944-954

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Abstract

The incidence of testicular cancer is increasing worldwide. Leydig cell tumors represent one type of sex cord‑stromal testis malignancy, which tend to respond unfavorably to chemotherapies. Identifying more efficient treatment strategies is therefore crucial for patients. The present study aimed to investigate the apoptotic effects of arsenic compounds and their underlying mechanisms. The results indicated that sodium arsenite and dimethylarsenic acid induced apoptosis of the murine Leydig tumor cell line, MA‑10. These apoptotic effects were characterized morphologically by membrane blebbing and cell detachment assays, biochemically using a cell viability assay, and cytologically by flow cytometry analysis. Western blotting demonstrated that caspases‑3, ‑8 and ‑9, and poly(ADP‑ribose) polymerase protein levels were increased compared with untreated MA‑10 cells; however, the caspase inhibitor, Z‑VAD‑fmk, reversed these effects. In conclusion, the present study has shown that sodium arsenite and dimethylarsenic acid may activate the intrinsic and extrinsic caspase pathways, and induce MA‑10 cell apoptosis. These results suggest that sodium arsenite and dimethylarsenic acid may represent novel approaches to treat clinically unmanageable forms of testicular cancer.

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