Inhibition of MCF-7 breast cancer cell-induced platelet aggregation using a combination of antiplatelet drugs

  • Authors:
    • Lian Lian
    • Wei Li
    • Zhen-Yu Li
    • Yi-Xiang Mao
    • You-Tao Zhang
    • Yi-Ming Zhao
    • Kai Chen
    • Wei-Ming Duan
    • Min Tao
  • View Affiliations

  • Published online on: December 13, 2012     https://doi.org/10.3892/ol.2012.1074
  • Pages: 675-680
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Abstract

Cancer metastasis is a highly coordinated and dynamic multistep process in which cancer cells interact with a variety of host cells. Morphological studies have documented the association of circulating tumor cells with host platelets. Tumor cell-induced platelet aggregation (TCIPA) contributes significantly to hematogenous metastasis; however, the molecular mechanisms involved in breast cancer TCIPA are poorly characterized. In this study, MCF-7 metastatic human breast cancer cells induced dose-dependent aggregation of washed platelets. Four major platelet activation pathways, glycoprotein (GP)-Ib-IX, GPIIb/IIIa, thromboxane (TX)-A2 and adenosine diphosphate (ADP) were activated during TCIPA and were inhibited by their respective inhibitors, 7E3, SZ-1, aspirin and apyrase. Pretreatment of platelets with 7E3, SZ-1 or apyrase significantly inhibited TCIPA, while pretreatment with aspirin had no effect. Moreover, combined pretreatment of platelets with 7E3, SZ-1 and apyrase significantly inhibited TCIPA, compared to single inhibitors. Combinations of antiplatelet drugs may represent a promising strategy to prevent cancer metastasis.
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February 2013
Volume 5 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Lian L, Li W, Li Z, Mao Y, Zhang Y, Zhao Y, Chen K, Duan W and Tao M: Inhibition of MCF-7 breast cancer cell-induced platelet aggregation using a combination of antiplatelet drugs. Oncol Lett 5: 675-680, 2013.
APA
Lian, L., Li, W., Li, Z., Mao, Y., Zhang, Y., Zhao, Y. ... Tao, M. (2013). Inhibition of MCF-7 breast cancer cell-induced platelet aggregation using a combination of antiplatelet drugs. Oncology Letters, 5, 675-680. https://doi.org/10.3892/ol.2012.1074
MLA
Lian, L., Li, W., Li, Z., Mao, Y., Zhang, Y., Zhao, Y., Chen, K., Duan, W., Tao, M."Inhibition of MCF-7 breast cancer cell-induced platelet aggregation using a combination of antiplatelet drugs". Oncology Letters 5.2 (2013): 675-680.
Chicago
Lian, L., Li, W., Li, Z., Mao, Y., Zhang, Y., Zhao, Y., Chen, K., Duan, W., Tao, M."Inhibition of MCF-7 breast cancer cell-induced platelet aggregation using a combination of antiplatelet drugs". Oncology Letters 5, no. 2 (2013): 675-680. https://doi.org/10.3892/ol.2012.1074