Effect of autophagy inhibition on chemotherapy‑induced apoptosis in A549 lung cancer cells

  • Authors:
    • Feifei Liu
    • Donglei Liu
    • Yang Yang
    • Song Zhao
  • View Affiliations

  • Published online on: January 25, 2013     https://doi.org/10.3892/ol.2013.1154
  • Pages: 1261-1265
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Abstract

Chemotherapy is one of the main methods of cancer treatment and is known to induce autophagy in cancer cells. The main mechanism of chemotherapeutic agents is to promote apoptosis. In the process of chemotherapy, there is a unique association between autophagy and apoptosis. In this study, MDC staining, Hoechst 33342 staining and flow cytometry were used to explore the effects of autophagy on chemotherapy-induced apoptosis in A549 lung cancer cells and the association between autophagy and apoptosis was investigated via the addition of an autophagic inhibitor (3-methyladenine, 3-MA). This study demonstrated that cisplatin and paclitaxel were able to induce autophagy and apoptosis in A549 lung cancer cells and the inhibition of autophagy promoted cisplatin and paclitaxel‑induced apoptosis. Furthermore, autophagy may play a protective role in the processes of cisplatin and paclitaxel-induced apoptosis.
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April 2013
Volume 5 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Liu F, Liu D, Yang Y and Zhao S: Effect of autophagy inhibition on chemotherapy‑induced apoptosis in A549 lung cancer cells. Oncol Lett 5: 1261-1265, 2013.
APA
Liu, F., Liu, D., Yang, Y., & Zhao, S. (2013). Effect of autophagy inhibition on chemotherapy‑induced apoptosis in A549 lung cancer cells. Oncology Letters, 5, 1261-1265. https://doi.org/10.3892/ol.2013.1154
MLA
Liu, F., Liu, D., Yang, Y., Zhao, S."Effect of autophagy inhibition on chemotherapy‑induced apoptosis in A549 lung cancer cells". Oncology Letters 5.4 (2013): 1261-1265.
Chicago
Liu, F., Liu, D., Yang, Y., Zhao, S."Effect of autophagy inhibition on chemotherapy‑induced apoptosis in A549 lung cancer cells". Oncology Letters 5, no. 4 (2013): 1261-1265. https://doi.org/10.3892/ol.2013.1154