Open Access

Genomic and phenotypic profiles of two Brazilian breast cancer cell lines derived from primary human tumors

  • Authors:
    • Natássia C.R. Corrêa
    • Hellen Kuasne
    • Jerusa A.Q.A. Faria
    • Ciça C.S. Seixas
    • Iria G.D. Santos
    • Francine B. Abreu
    • Suely Nonogaki
    • Rafael M. Rocha
    • Gerluza Aparecida Borges Silva
    • Helenice Gobbi
    • Silvia R. Rogatto
    • Alfredo M. Goes
    • Dawidson A. Gomes
  • View Affiliations

  • Published online on: February 12, 2013     https://doi.org/10.3892/or.2013.2284
  • Pages: 1299-1307
  • Copyright: © Corrêa et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Breast cancer is the most common type of cancer among women worldwide. Research using breast cancer cell lines derived from primary tumors may provide valuable additional knowledge regarding this type of cancer. Therefore, the aim of this study was to investigate the phenotypic profiles of MACL-1 and MGSO-3, the only Brazilian breast cancer cell lines available for comparative studies. We evaluated the presence of hormone receptors, proliferation, differentiation and stem cell markers, using immunohistochemical staining of the primary tumor, cultured cells and xenografts implanted in immunodeficient mice. We also investigated the ability of the cell lines to form colonies and copy number alterations by array comparative genomic hybridization. Histopathological analysis showed that the invasive primary tumor from which the MACL-1 cell line was derived, was a luminal A subtype carcinoma, while the ductal carcinoma in situ (DCIS) that gave rise to the MGSO-3 cell line was a HER2 subtype tumor, both showing different proliferation levels. The cell lines and the tumor xenografts in mice preserved their high proliferative potential, but did not maintain the expression of the other markers assessed. This shift in expression may be due to the selection of an ‘establishment’ phenotype in vitro. Whole-genome DNA evaluation showed a large amount of copy number alterations (CNAs) in the two cell lines. These findings render MACL-1 and MGSO-3 the first characterized Brazilian breast cancer cell lines to be potentially used for comparative research.
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April 2013
Volume 29 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Corrêa NC, Kuasne H, Faria JA, Seixas CC, Santos IG, Abreu FB, Nonogaki S, Rocha RM, Aparecida Borges Silva G, Gobbi H, Gobbi H, et al: Genomic and phenotypic profiles of two Brazilian breast cancer cell lines derived from primary human tumors. Oncol Rep 29: 1299-1307, 2013.
APA
Corrêa, N.C., Kuasne, H., Faria, J.A., Seixas, C.C., Santos, I.G., Abreu, F.B. ... Gomes, D.A. (2013). Genomic and phenotypic profiles of two Brazilian breast cancer cell lines derived from primary human tumors. Oncology Reports, 29, 1299-1307. https://doi.org/10.3892/or.2013.2284
MLA
Corrêa, N. C., Kuasne, H., Faria, J. A., Seixas, C. C., Santos, I. G., Abreu, F. B., Nonogaki, S., Rocha, R. M., Aparecida Borges Silva, G., Gobbi, H., Rogatto, S. R., Goes, A. M., Gomes, D. A."Genomic and phenotypic profiles of two Brazilian breast cancer cell lines derived from primary human tumors". Oncology Reports 29.4 (2013): 1299-1307.
Chicago
Corrêa, N. C., Kuasne, H., Faria, J. A., Seixas, C. C., Santos, I. G., Abreu, F. B., Nonogaki, S., Rocha, R. M., Aparecida Borges Silva, G., Gobbi, H., Rogatto, S. R., Goes, A. M., Gomes, D. A."Genomic and phenotypic profiles of two Brazilian breast cancer cell lines derived from primary human tumors". Oncology Reports 29, no. 4 (2013): 1299-1307. https://doi.org/10.3892/or.2013.2284