Anticancer effects of β-elemene in gastric cancer cells and its potential underlying proteins: A proteomic study

  • Authors:
    • Jun-Song Liu
    • Shi-Cai He
    • Zheng-Liang Zhang
    • Rui Chen
    • Lin Fan
    • Guang‑Lin Qiu
    • Shuai Chang
    • Liang Li
    • Xiang-Ming Che
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  • Published online on: September 17, 2014     https://doi.org/10.3892/or.2014.3490
  • Pages: 2635-2647
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Abstract

Gastric cancer is a common malignancy with a poor prognosis. β-elemene is a broad-spectrum anticancer drug extracted from the traditional Chinese medicinal herb Curcuma wenyujin. In the present study, we investigated the anticancer effects of β-elemene in gastric cancer cells and the potential proteins involved. Human SGC7901 and MKN45 gastric cancer cells were treated with different concentrations of β-elemene. Cell viability, clonogenic survival and apoptotic cell death were assessed. β-elemene inhibited viability and decreased clonogenic survival of gastric cancer cells in a dose-dependent manner. Apoptosis induction contributed to the anticancer effects. We then employed a proteomic method, isobaric tags for relative and absolute quantitation (iTRAQ), to detect the proteins altered by β-elemene. In total, 147 upregulated proteins and 86 downregulated proteins were identified in response to β-elemene treatment in SGC7901 gastric cancer cells. Among them, expression of p21-activated protein kinase‑interacting protein 1 (PAK1IP1), Bcl-2-associated transcription factor 1 (BTF) and topoisomerase 2-α (TOPIIα) were validated by western blot analyses and the trends were consistent with iTRAQ results. Top pathways involved in β-elemene treatment in SGC7901 gastric cancer cells included ribosome signaling, peroxisome proliferator-activated receptors (PPARs) signaling pathway, regulation of actin cytoskeleton, phagosome, biosynthesis and metabolism of some amino acids. Collectively, our results suggest a promising therapeutic role of β-elemene in gastric cancer. The differentially expressed proteins provide further insight into the potential mechanisms involved in gastric cancer treatment using β-elemene.
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December-2014
Volume 32 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Liu J, He S, Zhang Z, Chen R, Fan L, Qiu GL, Chang S, Li L and Che X: Anticancer effects of β-elemene in gastric cancer cells and its potential underlying proteins: A proteomic study. Oncol Rep 32: 2635-2647, 2014
APA
Liu, J., He, S., Zhang, Z., Chen, R., Fan, L., Qiu, G. ... Che, X. (2014). Anticancer effects of β-elemene in gastric cancer cells and its potential underlying proteins: A proteomic study. Oncology Reports, 32, 2635-2647. https://doi.org/10.3892/or.2014.3490
MLA
Liu, J., He, S., Zhang, Z., Chen, R., Fan, L., Qiu, G., Chang, S., Li, L., Che, X."Anticancer effects of β-elemene in gastric cancer cells and its potential underlying proteins: A proteomic study". Oncology Reports 32.6 (2014): 2635-2647.
Chicago
Liu, J., He, S., Zhang, Z., Chen, R., Fan, L., Qiu, G., Chang, S., Li, L., Che, X."Anticancer effects of β-elemene in gastric cancer cells and its potential underlying proteins: A proteomic study". Oncology Reports 32, no. 6 (2014): 2635-2647. https://doi.org/10.3892/or.2014.3490