Effects of the suppression of lactate dehydrogenase A on the growth and invasion of human gastric cancer cells

  • Authors:
    • Xiaojun Liu
    • Zhongxia Yang
    • Zhaofeng Chen
    • Rui Chen
    • Da Zhao
    • Yongning Zhou
    • Liang Qiao
  • View Affiliations

  • Published online on: November 12, 2014     https://doi.org/10.3892/or.2014.3600
  • Pages: 157-162
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Abstract

Lactate dehydrogenase A (LDH-A), which regulates glycolytic flux by catalyzing pyruvate to lactate in the cytoplasm, is believed to be one of the highly attractive therapeutic targets for cancers. Firstly, we detected the expression of LDH-A in gastric cancer (GC) cells. LDH-A inhibitor oxamate was then used to suppress the LDH-A activity in GC cells. Cell proliferation, lactic acid production, Transwell migration assay and apoptosis were assessed, respectively. The results showed that inhibition of LDH-A by oxamate decreased the lactate production. In the presence of glucose, oxamate inhibited cell proliferation in a dose-dependent manner. Flow cytometry assay further confirmed a pro-apoptotic effect of oxamate, and this was likely through increased expression of Bax, activated caspase-3, and decreased expression of Bcl-2. Therefore, we believe that oxamate inhibits cell growth, suppresses tumor invasion, and induces apoptosis in GC cells. LDH-A may be a potential therapeutic target for GC.

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January 2015
Volume 33 Issue 1

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APA
Liu, X., Yang, Z., Chen, Z., Chen, R., Zhao, D., Zhou, Y., & Qiao, L. (2015). Effects of the suppression of lactate dehydrogenase A on the growth and invasion of human gastric cancer cells. Oncology Reports, 33, 157-162. https://doi.org/10.3892/or.2014.3600
MLA
Liu, X., Yang, Z., Chen, Z., Chen, R., Zhao, D., Zhou, Y., Qiao, L."Effects of the suppression of lactate dehydrogenase A on the growth and invasion of human gastric cancer cells". Oncology Reports 33.1 (2015): 157-162.
Chicago
Liu, X., Yang, Z., Chen, Z., Chen, R., Zhao, D., Zhou, Y., Qiao, L."Effects of the suppression of lactate dehydrogenase A on the growth and invasion of human gastric cancer cells". Oncology Reports 33, no. 1 (2015): 157-162. https://doi.org/10.3892/or.2014.3600