miR-142 acts as a tumor suppressor in osteosarcoma cell lines by targeting Rac1

  • Authors:
    • Zhonghui Zheng
    • Muliang Ding
    • Jiangdong Ni
    • Deye Song
    • Jun Huang
    • Junjie Wang
  • View Affiliations

  • Published online on: December 22, 2014     https://doi.org/10.3892/or.2014.3687
  • Pages:1291-1299
0

Abstract

Although the 5-year survival rate of osteosarcoma (OS) has risen to ~60-70%, a substantial portion of patients still respond poorly to chemotherapy and have a high risk of relapse or metastasis even after curative resection. In this study, we found that the expression of miR-142 was significantly reduced in OS tissues and OS cell lines, while Ras-related C3 botulinum toxin substrate 1 (Rac1) expression was increased in the OS tissues and OS cell lines compared with expression in the controls. We then demonstrated that miR-142 regulated Rac1 expression at the transcriptional and translational levels by directly targeting its 3'-untranslated region (3'UTR). In addition, by loss- and gain-of function experiments, we investigated the role of miR-142 in OS cell lines and found that miR-142 acted as a tumor suppressor in the OS cell lines and inhibited cell proliferation and cell invasion and arrested cell cycle in the S phase. Furthermore, miR-142 inhibited osteosarcoma cell invasion by inducing E-cadherin expression and reducing expression of matrix metalloproteinase 2 (MMP2) and MMP9. Thus, overexpression of miR-142 and/or knockdown of Rac1 would be a novel target for OS therapy in the future.

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March 2015
Volume 33 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

2015 Impact Factor: 2.486
Ranked #31/211 Oncology
(total number of cites)

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APA
Zheng, Z., Ding, M., Ni, J., Song, D., Huang, J., & Wang, J. (2015). miR-142 acts as a tumor suppressor in osteosarcoma cell lines by targeting Rac1. Oncology Reports, 33, 1291-1299. https://doi.org/10.3892/or.2014.3687
MLA
Zheng, Z., Ding, M., Ni, J., Song, D., Huang, J., Wang, J."miR-142 acts as a tumor suppressor in osteosarcoma cell lines by targeting Rac1". Oncology Reports 33.3 (2015): 1291-1299.
Chicago
Zheng, Z., Ding, M., Ni, J., Song, D., Huang, J., Wang, J."miR-142 acts as a tumor suppressor in osteosarcoma cell lines by targeting Rac1". Oncology Reports 33, no. 3 (2015): 1291-1299. https://doi.org/10.3892/or.2014.3687