CD151 knockdown inhibits osteosarcoma metastasis through the GSK-3β/β-catenin/MMP9 pathway

  • Authors:
    • Zhiyu Zhang
    • Feng Wang
    • Qin Li
    • Haifei Zhang
    • Yan Cui
    • Chengbin Ma
    • Jiajun Zhu
    • Xueyuan Gu
    • Zhenguo Sun
  • View Affiliations

  • Published online on: December 24, 2015     https://doi.org/10.3892/or.2015.4517
  • Pages: 1764-1770
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Abstract

Osteosarcoma (OS) is a primary bone malignancy with a high early metastatic propensity. It is crucial to find specific protein targets to develop therapeutic strategies against this lethal disease. Tetraspanin CD151 is involved in facilitating tumor metastasis. However, the role and molecular mechanism of CD151 in promoting OS metastasis remain enigmatic. In the present study, we used small interfering RNA (siRNA) to inhibit CD151 expression in highly metastatic OS cells and the results demonstrated that CD151 knockdown inhibited their migration, invasion and metastasis. We further investigated the molecular mechanism of CD151 by inhibiting genes known to be involved in metastasis in OS cells and found that CD151 modulated matrix metalloproteinase 9 (MMP9) expression through the glycogen synthase kinase 3 (GSK-3β)/β-catenin signaling pathway. We conclude that CD151 knockdown inhibits the expression of MMP9 through the GSK-3β/β‑catenin pathway and also inhibits OS migration and invasion in vitro and metastasis in vivo in highly metastatic OS. This suggests that CD151 may be a useful antimetastatic target for OS.
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March-2016
Volume 35 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhang Z, Wang F, Li Q, Zhang H, Cui Y, Ma C, Zhu J, Gu X and Sun Z: CD151 knockdown inhibits osteosarcoma metastasis through the GSK-3β/β-catenin/MMP9 pathway. Oncol Rep 35: 1764-1770, 2016
APA
Zhang, Z., Wang, F., Li, Q., Zhang, H., Cui, Y., Ma, C. ... Sun, Z. (2016). CD151 knockdown inhibits osteosarcoma metastasis through the GSK-3β/β-catenin/MMP9 pathway. Oncology Reports, 35, 1764-1770. https://doi.org/10.3892/or.2015.4517
MLA
Zhang, Z., Wang, F., Li, Q., Zhang, H., Cui, Y., Ma, C., Zhu, J., Gu, X., Sun, Z."CD151 knockdown inhibits osteosarcoma metastasis through the GSK-3β/β-catenin/MMP9 pathway". Oncology Reports 35.3 (2016): 1764-1770.
Chicago
Zhang, Z., Wang, F., Li, Q., Zhang, H., Cui, Y., Ma, C., Zhu, J., Gu, X., Sun, Z."CD151 knockdown inhibits osteosarcoma metastasis through the GSK-3β/β-catenin/MMP9 pathway". Oncology Reports 35, no. 3 (2016): 1764-1770. https://doi.org/10.3892/or.2015.4517