BLCAP arrests G1/S checkpoint and induces apoptosis through downregulation of pRb1 in HeLa cells

  • Authors:
    • Min Zhao
    • Li Zhang
    • Xiaoping Qiu
    • Fanyu Zeng
    • Wen Chen
    • Yuehui An
    • Bicheng Hu
    • Xufeng Wu
    • Xinxing Wu
  • View Affiliations

  • Published online on: March 17, 2016     https://doi.org/10.3892/or.2016.4686
  • Pages: 3050-3058
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Abstract

BLCAP (bladder cancer-associated protein) gene exhibited tumor suppressor function in different tumors and is regarded as a candidate tumor suppressor gene; however, the mechanism by which BLCAP exerts its function remains elusive. This study investigated the functional association between BLCAP and proliferation or apoptosis in cervical cancer cells, to identify the functional motifs of BLCAP. The BLCAP-shRNA expression vector based on pRNA-U6.1/Hygro plasmid was used to specifically inhibit BLCAP activity in HeLa cells. The optimal shRNA plasmid was selected to knock down BLCAP expression and the biological effects were investigated. The effects on cell cycle and apoptosis were detected by flow cytometric or Annexin V-FITC staining analysis. The gene expression profiles of HeLa cells transfected with blcap-wt and BLCAP-shRNA were analyzed using human signal pathway gene Oligochips. The levels of protein expression and interaction of BLCAP with Rb1 proteins were determined by western blotting and Co-IP assays. The site-specific mutagenesis assay was used to identify amino acid residues important for BLCAP. Significantly differentially expressed genes were found by gene Oligo chips analysis. These genes were all correlated with proliferation, cell cycle and apoptosis. The results of western blotting and Co-IP assays confirmed that overexpression of BLCAP could interact with Rb1 and inhibit Rb1 phosphorylation. Further investigation revealed that SAXX mutation in the key regions of BLCAP suppressed the function of BLCAP and significantly increased the level of phosphorylated Rb1 protein. Here our findings suggested that the functional association of BLCAP and Rb1 might play important roles in proliferation and apoptosis of HeLa cells. It suggested that BLCAP could be a novel therapeutic target for cervical cancer.
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May-2016
Volume 35 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhao M, Zhang L, Qiu X, Zeng F, Chen W, An Y, Hu B, Wu X and Wu X: BLCAP arrests G1/S checkpoint and induces apoptosis through downregulation of pRb1 in HeLa cells. Oncol Rep 35: 3050-3058, 2016.
APA
Zhao, M., Zhang, L., Qiu, X., Zeng, F., Chen, W., An, Y. ... Wu, X. (2016). BLCAP arrests G1/S checkpoint and induces apoptosis through downregulation of pRb1 in HeLa cells. Oncology Reports, 35, 3050-3058. https://doi.org/10.3892/or.2016.4686
MLA
Zhao, M., Zhang, L., Qiu, X., Zeng, F., Chen, W., An, Y., Hu, B., Wu, X., Wu, X."BLCAP arrests G1/S checkpoint and induces apoptosis through downregulation of pRb1 in HeLa cells". Oncology Reports 35.5 (2016): 3050-3058.
Chicago
Zhao, M., Zhang, L., Qiu, X., Zeng, F., Chen, W., An, Y., Hu, B., Wu, X., Wu, X."BLCAP arrests G1/S checkpoint and induces apoptosis through downregulation of pRb1 in HeLa cells". Oncology Reports 35, no. 5 (2016): 3050-3058. https://doi.org/10.3892/or.2016.4686