Crocodile blood extract induces the apoptosis of lung cancer cells through PTEN activity

  • Authors:
    • Yuqian Ou
    • Wing Shing Ho
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  • Published online on: July 5, 2016     https://doi.org/10.3892/or.2016.4914
  • Pages: 1457-1466
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Abstract

Current treatment strategies for lung cancer cause undesirable side‑effects. Integrated medicine with a curative approach has become a common approach to the treatment strategy. Recent studies suggest that American alligator blood is effective in reducing colorectal cancer cell viability in vitro, but the mechanism remains unclear. In the present study, we aimed to study the anticancer activity of crocodile blood extracts on lung cancer cell line A549 and investigate the possible mechanisms involved. In vitro studies were utilized to investigate the effects on the cancer cells after incubation with the blood extracts. The active fraction that showed more efficacy in inhibiting cell growth was characterized in the supernatant (S2) from whole blood extracts. High performance liquid chromatography (HPLC) analysis revealed that S2 contained more polar moiety from whole blood. S2 induced DNA fragmentation. Cell cycle arrest in the G1/M phase was demonstrated and mitochondrial membrane permeability was disrupted. An increase in the generation of reactive oxygen species (ROS) and increased activities of caspase-3 and caspase-7 were detected. Furthermore, release of cytochrome c, upregulation of expression of Bax, p53, p21, Bid, cleaved forms of the caspase family and PARP along with downregulation of Bcl-2, PCNA, MDM2, caspase‑8, wild types of caspase family proteins and PARP were recorded after treatment with S2 fractions. Moreover, the PI3K/AKT survival pathway was downregulated by S2 fractions in the lung cancer cell line.
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September-2016
Volume 36 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ou Y and Ho WS: Crocodile blood extract induces the apoptosis of lung cancer cells through PTEN activity. Oncol Rep 36: 1457-1466, 2016.
APA
Ou, Y., & Ho, W.S. (2016). Crocodile blood extract induces the apoptosis of lung cancer cells through PTEN activity. Oncology Reports, 36, 1457-1466. https://doi.org/10.3892/or.2016.4914
MLA
Ou, Y., Ho, W. S."Crocodile blood extract induces the apoptosis of lung cancer cells through PTEN activity". Oncology Reports 36.3 (2016): 1457-1466.
Chicago
Ou, Y., Ho, W. S."Crocodile blood extract induces the apoptosis of lung cancer cells through PTEN activity". Oncology Reports 36, no. 3 (2016): 1457-1466. https://doi.org/10.3892/or.2016.4914