CCL22 and IL-37 inhibit the proliferation and epithelial-mesenchymal transition process of NSCLC A549 cells

Corrigendum in: /10.3892/or.2020.7903

  • Authors:
    • Yu-Hua Chen
    • Bi-Yun Zhou
    • Xian-Jing Wu
    • Jun-Fa Xu
    • Jun‑Ai Zhang
    • Yong-Hua Chen
    • Si-Si Liang
  • View Affiliations

  • Published online on: August 2, 2016     https://doi.org/10.3892/or.2016.4995
  • Pages: 2017-2024
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Abstract

In the present study, we aimed to investigate the effects of CC chemokine ligand 22 (CCL22) and interleukin-37 (IL-37) on the proliferation and epithelial-mesenchymal transition (EMT) of non-small cell lung cancer (NSCLC) A549 cells. pDsRed-CCL22 and pEGFP-IL-37 plasmids were constructed. A549 cells were divided into six groups: the control, the pDsRed-N1 blank plasmid, the pEGFP-C1 blank plasmid, the pDsRed-CCL22 plasmid, the pEGFP‑IL-37 plasmid and the pDsRed-CCL22 + pEGFP-IL-37 plasmid group. Expression levels and localization of CCL22 and IL-37 in cells were detected by confocal microscopy. Phase-contrast microscopy was applied for observing cellular morphology. Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) was used for detecting the mRNA levels of vimentin, N-cadherin and E-cadherin, and their protein expression levels were tested using western blotting. Constructed plasmids expressed CCL22 and IL-37, both of which had a co-localization in the cell membrane. MTT assay and cell observation results revealed that CCL22 and IL-37 inhibited the proliferation and EMT process of the A549 cells. The results of RT-qPCR and western blotting revealed that decreased vimentin and N-cadherin mRNA and protein expression levels, and increased E-cadherin mRNA and protein expression levels were found in the pDsRed-CCL22 plasmid, pEGFP-IL-37 plasmid and pDsRed‑CCL22 + pEGFP‑IL-37 plasmid groups when compared with the control, the pDsRed-N1 blank plasmid and the pEGFP-C1 blank plasmid groups (all P<0.05), and decreased vimentin and N-cadherin mRNA and protein expression levels and increased E-cadherin mRNA and protein expression levels were found in the pDsRed‑CCL22 + pEGFP‑IL-37 plasmid group when compared with the pDsRed-CCL22 plasmid and the pEGFP‑IL-37 plasmid groups (all P<0.05). CCL22 and IL-37 with a co-localization in the A549 cells inhibited the proliferation and EMT process in A549 cells. The antitumor effects of CCL22 and IL37 provide a strategy for the treatment of NSCLC.
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October-2016
Volume 36 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Chen Y, Zhou B, Wu X, Xu J, Zhang JA, Chen Y and Liang S: CCL22 and IL-37 inhibit the proliferation and epithelial-mesenchymal transition process of NSCLC A549 cells Corrigendum in /10.3892/or.2020.7903. Oncol Rep 36: 2017-2024, 2016.
APA
Chen, Y., Zhou, B., Wu, X., Xu, J., Zhang, J., Chen, Y., & Liang, S. (2016). CCL22 and IL-37 inhibit the proliferation and epithelial-mesenchymal transition process of NSCLC A549 cells Corrigendum in /10.3892/or.2020.7903. Oncology Reports, 36, 2017-2024. https://doi.org/10.3892/or.2016.4995
MLA
Chen, Y., Zhou, B., Wu, X., Xu, J., Zhang, J., Chen, Y., Liang, S."CCL22 and IL-37 inhibit the proliferation and epithelial-mesenchymal transition process of NSCLC A549 cells Corrigendum in /10.3892/or.2020.7903". Oncology Reports 36.4 (2016): 2017-2024.
Chicago
Chen, Y., Zhou, B., Wu, X., Xu, J., Zhang, J., Chen, Y., Liang, S."CCL22 and IL-37 inhibit the proliferation and epithelial-mesenchymal transition process of NSCLC A549 cells Corrigendum in /10.3892/or.2020.7903". Oncology Reports 36, no. 4 (2016): 2017-2024. https://doi.org/10.3892/or.2016.4995