Regulation of DEK expression by AP-2α and methylation level of DEK promoter in hepatocellular carcinoma

  • Authors:
    • Ming-Xu Qiao
    • Chun Li
    • Ai-Qun Zhang
    • Ling-Ling Hou
    • Juan Yang
    • Hong-Gang Hu
  • View Affiliations

  • Published online on: July 28, 2016     https://doi.org/10.3892/or.2016.4984
  • Pages: 2382-2390
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Abstract

DEK is overexpressed in multiple invasive tumors. However, the transcriptional regulatory mechanism of DEK remains unclear. In the present study, progressive-type truncation assay indicated that CpG2-2 (-167 bp/+35 bp) was the DEK core promoter, whose methylation inhibited DEK expression. Bisulfite genomic sequencing analysis indicated that the methylation levels of the DEK promoter in normal hepatic cells and tissues were higher than those in hepatocellular carcinoma (HCC) cells. TFSEARCH result revealed transcription factor binding sites in CpG2-2. Among the sites, the AP-2α binding site showed the most significant methylation difference; hence, AP-2α is a key transcription factor that regulates DEK expression. Point or deletion mutation of the AP-2α binding site significantly reduced the promoter activity. Chromatin immunoprecipitation assay demonstrated the binding of AP-2α to the core promoter. Furthermore, knock down of endogenous AP-2α downregulated DEK expression, whereas overexpression of AP-2α upregulated DEK expression. Thus, AP-2α is an important transcription factor of DEK expression, which is correlated with the methylation level of the DEK core promoter in HCC.
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October-2016
Volume 36 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Qiao M, Li C, Zhang A, Hou L, Yang J and Hu H: Regulation of DEK expression by AP-2α and methylation level of DEK promoter in hepatocellular carcinoma. Oncol Rep 36: 2382-2390, 2016
APA
Qiao, M., Li, C., Zhang, A., Hou, L., Yang, J., & Hu, H. (2016). Regulation of DEK expression by AP-2α and methylation level of DEK promoter in hepatocellular carcinoma. Oncology Reports, 36, 2382-2390. https://doi.org/10.3892/or.2016.4984
MLA
Qiao, M., Li, C., Zhang, A., Hou, L., Yang, J., Hu, H."Regulation of DEK expression by AP-2α and methylation level of DEK promoter in hepatocellular carcinoma". Oncology Reports 36.4 (2016): 2382-2390.
Chicago
Qiao, M., Li, C., Zhang, A., Hou, L., Yang, J., Hu, H."Regulation of DEK expression by AP-2α and methylation level of DEK promoter in hepatocellular carcinoma". Oncology Reports 36, no. 4 (2016): 2382-2390. https://doi.org/10.3892/or.2016.4984