Hyperthermic carbon dioxide pneumoperitoneum reinforces the inhibition of 5-FU on the proliferation and invasion of colon cancer

Zhao,Jiaying; Lv,You; Cai,Yuankun; Wei,Wangui; Yin,Chenqing; Wang,Xin; Hao,Zong; Shen,Chenxia; Wang,Huipeng

doi:10.3892/or.2016.5229

Hyperthermic carbon dioxide pneumoperitoneum reinforces the inhibition of 5-FU on the proliferation and invasion of colon cancer

Authors:
- Jiaying Zhao
- You Lv
- Yuankun Cai
- Wangui Wei
- Chenqing Yin
- Xin Wang
- Zong Hao
- Chenxia Shen
- Huipeng Wang
View Affiliations

Affiliations: Department of General Surgery, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China, Life Science Research Institute, Chinese Academy of Sciences, Shanghai 200031, P.R. China
Published online on: November 8, 2016 https://doi.org/10.3892/or.2016.5229
Pages: 492-500

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The effect of hyperthermic carbon dioxide (CO2) pneumoperitoneum in combination with 5-fluorouracil (5-FU) on the proliferation and invasion of colon cancer was explored. Colon cancer cell line SW-480 was sealed into the urine collection bag to simulate pneumoperitoneum with 100% CO2 under a pressure of 12 mmHg. The cells were divided into group A, CO2 at 37˚C; group B, CO2 at 43˚C; group C, 5-FU; group D, CO2 at 37˚C+5-FU; group E, CO2 at 43˚C+5-FU; and control groups under normal culture conditions. The cell proliferation was assessed by CCK-8 test; the cell apoptosis was tested by FACS analysis; the cell invasion was examined by Transwell assay; the expression of HSP-70, caspase-3, HIF-1α and MMP-9 proteins and genes were detected by western blot analysis and RT-PCR. The SW-480 cells were injected into nude mouse cecum subserosal to establish a colon cancer model. We applied 43˚C CO2 pneumoperitoneum or 5-FU intraperitoneal chemotherapy to intervene, detected the transplantation tumor growth and metastasis. The cell proliferation was inhibited in groups B, C, D and E, apoptosis was induced in groups B, C, D and E, the Transwell cell number decreased in groups B, C, D and E, the transplantation tumor weight and metastasis rate were inhibited in groups B, C, D and E, but all not in group A. The most significant change was observed in group E. Hyperthermic CO2 pneumoperitoneum was able to reinforce the inhibition of 5-FU on proliferation and invasion of colon cancer.

View Figures

View References

1	Bing F and Hong Z: The effect of CO₂ pneumoperitoneum on the growth and metastasis of malignant tumors of the rectum. J Exp Sur. 22:10212005.
2	Jingli C, Rong C and Rubai X: Influence of colorectal laparoscopic surgery on dissemination and seeding of tumor cells. Surg Endosc. 20:1759–1761. 2006. View Article : Google Scholar : PubMed/NCBI
3	Lin F, Pan L, Li L, Li D and Mo L: Effects of a simulated CO₂ pneumoperitoneum environment on the proliferation, apoptosis, and metastasis of cervical cancer cells in vitro. Med Sci Monit. 20:2497–2503. 2014. View Article : Google Scholar : PubMed/NCBI
4	Zhenggang Z: Comprehensive treatment and issues related to gastric cancer recurrence. Chin J Sur. 25:181–183. 2005.
5	Kodach LL, Bos CL, Durán N, Peppelenbosch MP, Ferreira CV and Hardwick JC: Violacein synergistically increases 5-fluorouracil cytotoxicity, induces apoptosis and inhibits Akt-mediated signal transduction in human colorectal cancer cells. Carcinogenesis. 27:508–516. 2006. View Article : Google Scholar : PubMed/NCBI
6	Zheng M, Lin S and Sun J: Impact of laparoscopic colorectal surgery on port-site and viscera metastasis. Chin J Pract Surg. 22:337–339. 2002.
7	Ceccarelli G, Casciola L, Nati S, Bartoli A, Spaziani A, Stefanoni M, Conti D, Fettucciari V, Di Zitti L, Valeri R, et al: Neoplastic residues in the trocar tract in oncologic laparoscopic surgery. Minerva Chir. 59:243–248. 2004.(In Italian). PubMed/NCBI
8	Jacobi CA, Ordemann J, Zieren HU and Müller JM: Effect of intra-abdominal pressure in laparoscopy on intraperitoneal tumor growth and development of trocar metastases. An animal experiment study in the rat model. Langenbecks Arch Chir Suppl Kongressbd. 115:(Suppl I). 529–533. 1998.[(In German)]. PubMed/NCBI
9	Wildbrett P, Oh A, Carter JJ, Schuster H, Bessler M, Jaboci CA and Whelan RL: Increased rates of pulmonary metastases following sham laparotomy compared to CO₂ pneumoperitoneum and the inhibition of metastases utilizing perioperative immunomodulation and a tumor vaccine. Surg Endosc. 16:1162–1169. 2002. View Article : Google Scholar : PubMed/NCBI
10	Buunen M, Veldkamp R, Hop WC, Kuhry E, Jeekel J, Haglind E, Påhlman L, Cuesta MA, Msika S, Morino M, et al: Colon Cancer Laparoscopic or Open Resection Study Group: Survival after laparoscopic surgery versus open surgery for colon cancer: Long-term outcome of a randomised clinical trial. Lancet Oncol. 10:44–52. 2009. View Article : Google Scholar : PubMed/NCBI
11	Hildebrandt B, Wust P, Ahlers O, Dieing A, Sreenivasa G, Kerner T, Felix R and Riess H: The cellular and molecular basis of hyperthermia. Crit Rev Oncol Hematol. 43:33–56. 2002. View Article : Google Scholar : PubMed/NCBI
12	Cai W, Dong F, Wang Z, Yang X, Zheng M and Che X: Heated and humidified CO₂ pneumoperitoneum inhibits tumour cell proliferation, migration and invasion in colon cancer. Int J Hyperthermia. 30:201–209. 2014. View Article : Google Scholar : PubMed/NCBI
13	Tabuchi Y, Wada S, Furusawa Y, Ohtsuka K and Kondo T: Gene networks related to the cell death elicited by hyperthermia in human oral squamous cell carcinoma HSC-3 cells. Int J Mol Med. 29:380–386. 2012.PubMed/NCBI
14	Al-Shammaa HA, Li Y and Yonemura Y: Current status and future strategies of cytoreductive surgery plus intraperitoneal hyperthermic chemotherapy for peritoneal carcinomatosis. World J Gastroenterol. 14:1159–1166. 2008. View Article : Google Scholar : PubMed/NCBI
15	Peng Y, Zheng M, Feng B, Chen X, Yu B, Lu A, Wang M, Li J, Ma J and Xu L: Hyperthermic CO₂ pneumoperitoneum induces apoptosis in human colon cancer cells through Bax-associated mitochondrial pathway. Oncol Rep. 19:73–79. 2008.PubMed/NCBI
16	Ito A, Shinkai M, Honda H, Yoshikawa K, Saga S, Wakabayashi T, Yoshida J and Kobayashi T: Heat shock protein 70 expression induces antitumor immunity during intracellular hyperthermia using magnetite nanoparticles. Cancer Immunol Immunother. 52:80–88. 2003.PubMed/NCBI
17	Depraetere V and Golstein P: Dismantling in cell death: Molecular mechanisms and relationship to caspase activation. Scand J Immunol. 47:523–531. 1998. View Article : Google Scholar : PubMed/NCBI
18	Zhu H, Feng Y, Zhang J, Zhou X, Hao B, Zhang G and Shi R: Inhibition of hypoxia inducible factor 1α expression suppresses the progression of esophageal squamous cell carcinoma. Cancer Biol Ther. 11:981–987. 2011. View Article : Google Scholar : PubMed/NCBI
19	Liu D, Guo H, Li Y, Xu X, Yang K and Bai Y: Association between polymorphisms in the promoter regions of matrix metalloproteinases (MMPs) and risk of cancer metastasis: A meta-analysis. PLoS One. 7:e312512012. View Article : Google Scholar : PubMed/NCBI
20	Chu D, Zhao Z, Zhou Y, Li Y, Li J, Zheng J, Zhao Q and Wang W: Matrix metalloproteinase-9 is associated with relapse and prognosis of patients with colorectal cancer. Ann Surg Oncol. 19:318–325. 2012. View Article : Google Scholar : PubMed/NCBI