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April-2017 Volume 37 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Epigenetics

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Article

Knockdown of GATAD2A suppresses cell proliferation in thyroid cancer in vitro

  • Authors:
    • Zongping Wang
    • Jie Kang
    • Xianzhao Deng
    • Bomin Guo
    • Bo Wu
    • Youben Fan
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The Sixth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200233, P.R. China
  • Pages: 2147-2152
    |
    Published online on: February 10, 2017
       https://doi.org/10.3892/or.2017.5436
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Abstract

GATAD2A (GATA zinc finger domain containing 2A), is a subunit of NuRP (nucleosome remodeling and histone deacetylation) which plays key roles in tumor growth inhibition and embryonic development. However, its role in thyroid cancer remains unclear. In our study, we established two thyroid cancer cell lines by lentivirus-delivered short hairpin (shRNA) to knockdown the expression of GATAD2A. Then loss-of-function assays indicated that knockdown of GATAD2A decreased the ability of cell proliferation and colony formation in thyroid cancer cells by MTT and colony formation assay, respectively. Moreover, cell cycle assay by flow cytometry revealed that the percentage of cells in G0/G1 phase was significantly decreased in GATAD2A knockdown cells accompanied by increase of cells in G2/M phase. Furthermore, inhibition of GATAD2A promoted cell apoptosis via elevating the expression of caspase-3 and PARP cleavage using Annexin V/7-AAD double staining and western blotting. In conclusion, GATAD2A is an essential factor in thyroid cancer cell growth and apoptosis, and may be a potential therapeutic biomarker in thyroid cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Z, Kang J, Deng X, Guo B, Wu B and Fan Y: Knockdown of GATAD2A suppresses cell proliferation in thyroid cancer in vitro. Oncol Rep 37: 2147-2152, 2017.
APA
Wang, Z., Kang, J., Deng, X., Guo, B., Wu, B., & Fan, Y. (2017). Knockdown of GATAD2A suppresses cell proliferation in thyroid cancer in vitro. Oncology Reports, 37, 2147-2152. https://doi.org/10.3892/or.2017.5436
MLA
Wang, Z., Kang, J., Deng, X., Guo, B., Wu, B., Fan, Y."Knockdown of GATAD2A suppresses cell proliferation in thyroid cancer in vitro". Oncology Reports 37.4 (2017): 2147-2152.
Chicago
Wang, Z., Kang, J., Deng, X., Guo, B., Wu, B., Fan, Y."Knockdown of GATAD2A suppresses cell proliferation in thyroid cancer in vitro". Oncology Reports 37, no. 4 (2017): 2147-2152. https://doi.org/10.3892/or.2017.5436
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Z, Kang J, Deng X, Guo B, Wu B and Fan Y: Knockdown of GATAD2A suppresses cell proliferation in thyroid cancer in vitro. Oncol Rep 37: 2147-2152, 2017.
APA
Wang, Z., Kang, J., Deng, X., Guo, B., Wu, B., & Fan, Y. (2017). Knockdown of GATAD2A suppresses cell proliferation in thyroid cancer in vitro. Oncology Reports, 37, 2147-2152. https://doi.org/10.3892/or.2017.5436
MLA
Wang, Z., Kang, J., Deng, X., Guo, B., Wu, B., Fan, Y."Knockdown of GATAD2A suppresses cell proliferation in thyroid cancer in vitro". Oncology Reports 37.4 (2017): 2147-2152.
Chicago
Wang, Z., Kang, J., Deng, X., Guo, B., Wu, B., Fan, Y."Knockdown of GATAD2A suppresses cell proliferation in thyroid cancer in vitro". Oncology Reports 37, no. 4 (2017): 2147-2152. https://doi.org/10.3892/or.2017.5436
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