RUNX3 plays a tumor suppressor role by inhibiting cell migration, invasion and angiogenesis in oral squamous cell carcinoma

  • Authors:
    • Wei-Na Zhou
    • Yi-Fei Du
    • Jin Bai
    • Xiao-Meng Song
    • Yang Zheng
    • Hua Yuan
    • Wei Zhang
    • Zheng-Dong Zhang
    • Yu-Nong Wu
  • View Affiliations

  • Published online on: July 31, 2017     https://doi.org/10.3892/or.2017.5857
  • Pages: 2378-2386
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Abstract

Although aberrant expression of Runt-related transcription factor 3 (RUNX3) contributes to tumor progression and metastasis in a number of carcinomas, the status of RUNX3 and its correlation with prognosis in oral squamous cell carcinomas (OSCC) are still controversial. The aim of present study was to investigate the function of RUNX3 in OSCC and the underlying molecular mechanisms. Tissue microarray (TMA) consisting of 232 OSCC specimens was used to detect the expression of RUNX3 by immunohistochemistry method. The effects of RUNX3 restoration on OSCC cell migration and invasion were determined by wound-healing assay, migration and Matrigel cell invasion assays. The antiangiogenic role of RUNX3 was analyzed by testing proliferation and tube formation of human umbilical vascular endothelial cells (HUVECs) cultured with conditioned medium from RUNX3 transfected OSCC cell lines. The activities of MMP-9 and VEGF in RUNX3 transfected OSCC cell lines were examined by western blot and Elisa methods. RUNX3 expression was reduced in OSCC specimens and significantly associated with tumor size (P=0.002), lymph node statue (P=0.0036) and clinical stage (P=0.0001). Negative expression of RUNX3 correlated with worse 5-year overall and disease-specific survival rates (P=0.0348 and P=0.0301, respectively). Furthermore, we found that RUNX3 restoration suppressed cell migration and invasion through downregulating MMP-9 expression and secretion, and exerted antiangiogenic capability by inhibiting VEGF activity in HN6 and Cal27 cells. These findings suggested that RUNX3 played a tumor suppressor role in OSCC by inhibiting cell migration, invasion and angiogenesis, supporting that it could be a potential therapeutic target for OSCC.
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October-2017
Volume 38 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhou W, Du Y, Bai J, Song X, Zheng Y, Yuan H, Zhang W, Zhang Z and Wu Y: RUNX3 plays a tumor suppressor role by inhibiting cell migration, invasion and angiogenesis in oral squamous cell carcinoma. Oncol Rep 38: 2378-2386, 2017.
APA
Zhou, W., Du, Y., Bai, J., Song, X., Zheng, Y., Yuan, H. ... Wu, Y. (2017). RUNX3 plays a tumor suppressor role by inhibiting cell migration, invasion and angiogenesis in oral squamous cell carcinoma. Oncology Reports, 38, 2378-2386. https://doi.org/10.3892/or.2017.5857
MLA
Zhou, W., Du, Y., Bai, J., Song, X., Zheng, Y., Yuan, H., Zhang, W., Zhang, Z., Wu, Y."RUNX3 plays a tumor suppressor role by inhibiting cell migration, invasion and angiogenesis in oral squamous cell carcinoma". Oncology Reports 38.4 (2017): 2378-2386.
Chicago
Zhou, W., Du, Y., Bai, J., Song, X., Zheng, Y., Yuan, H., Zhang, W., Zhang, Z., Wu, Y."RUNX3 plays a tumor suppressor role by inhibiting cell migration, invasion and angiogenesis in oral squamous cell carcinoma". Oncology Reports 38, no. 4 (2017): 2378-2386. https://doi.org/10.3892/or.2017.5857