miR-93 and PTEN: Key regulators of doxorubicin-resistance and EMT in breast cancer

  • Authors:
    • Shihua Chu
    • Geng Liu
    • Peixuan Xia
    • Guoqing Chen
    • Feng Shi
    • Tao Yi
    • Hongying Zhou
  • View Affiliations

  • Published online on: July 31, 2017     https://doi.org/10.3892/or.2017.5859
  • Pages: 2401-2407
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Abstract

It is not well established whether miR-93 is involved in drug resistance and epithelial-mesenchymal transition (EMT) in breast cancer, and its underlying mechanism remains uncertain. In the present study, the expression differences of miR-93 between paired breast cancer tissues confirmed it is involved in the progression of breast cancer. Such a difference was also observed in doxorubicin-resistant and -sensitive cells. Overexpressed miR-93 in sensitive cells revealed increases in cellular proliferation and the expression levels of drug-resistant-related genes, and a decrease in sensitivity to doxorubicin. This demonstrated the relationship between miR-93 and breast cancer drug resistance. Simultaneously, EMT was confirmed in miR-93 overexpressing sensitive cells. This indicated the triadic relationship among miR-93, EMT and drug resistance in breast cancer. We applied the Dual-luciferase Reporter assay to expose the direct interaction between miR-93 and PTEN, which suggested that miR-93 contributes to inducing EMT and drug resistance of breast cancer cells by targeting PTEN.
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October-2017
Volume 38 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Chu S, Liu G, Xia P, Chen G, Shi F, Yi T and Zhou H: miR-93 and PTEN: Key regulators of doxorubicin-resistance and EMT in breast cancer. Oncol Rep 38: 2401-2407, 2017
APA
Chu, S., Liu, G., Xia, P., Chen, G., Shi, F., Yi, T., & Zhou, H. (2017). miR-93 and PTEN: Key regulators of doxorubicin-resistance and EMT in breast cancer. Oncology Reports, 38, 2401-2407. https://doi.org/10.3892/or.2017.5859
MLA
Chu, S., Liu, G., Xia, P., Chen, G., Shi, F., Yi, T., Zhou, H."miR-93 and PTEN: Key regulators of doxorubicin-resistance and EMT in breast cancer". Oncology Reports 38.4 (2017): 2401-2407.
Chicago
Chu, S., Liu, G., Xia, P., Chen, G., Shi, F., Yi, T., Zhou, H."miR-93 and PTEN: Key regulators of doxorubicin-resistance and EMT in breast cancer". Oncology Reports 38, no. 4 (2017): 2401-2407. https://doi.org/10.3892/or.2017.5859