Comprehensive analysis of aberrantly expressed microRNA profiles reveals potential biomarkers of human lung adenocarcinoma progression

  • Authors:
    • Jing Sui
    • Ru-Song Yang
    • Si-Yi Xu
    • Yan-Qiu Zhang
    • Cheng-Yun Li
    • Sheng Yang
    • Li-Hong Yin
    • Yue-Pu Pu
    • Ge-Yu Liang
  • View Affiliations

  • Published online on: August 4, 2017     https://doi.org/10.3892/or.2017.5880
  • Pages: 2453-2463
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Abstract

Lung adenocarcinoma (LUAD) is a complex disease that poses challenges for diagnosis and treatment. The aim of the present study is to investigate LUAD-specific key microRNAs (miRNAs) from large-scale samples in The Cancer Genome Atlas (TCGA) database. We used an integrative computational method to identify LUAD-specific key miRNAs related to TNM stage and lymphatic metastasis from the TCGA database. Twenty-five LUAD-specific key miRNAs (fold change >2, p<0.05) from the TCGA database were investigated, and 15 were found to be aberrantly expressed with respect to clinical features. Three miRNAs were correlated with overall survival (log-rank p<0.05). Then, 5 miRNAs were randomly selected for verification of expression in 53 LUAD patient tissues using qRT-PCR. Diagnostic value of these above 5 miRNAs was determined by areas under receiver operating characteristic curves (ROC). Finally, the LUAD-related miRNA miR-30a-3p was selected for verification of biologic function in A549 cells. The results of tests for cell proliferation, apoptosis, and target genes suggested that miR-30a-3p decreases cell proliferation and promotes apoptosis through targeting AKT3. Therefore, miR-30a-3p may be a promising biomarker for the early screening of high-risk populations and early diagnosis of LUAD. Our studies provide insights into identifying novel potential biomarkers for diagnosis and prognosis of LUAD.
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October-2017
Volume 38 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Sui J, Yang R, Xu S, Zhang Y, Li C, Yang S, Yin L, Pu Y and Liang G: Comprehensive analysis of aberrantly expressed microRNA profiles reveals potential biomarkers of human lung adenocarcinoma progression. Oncol Rep 38: 2453-2463, 2017
APA
Sui, J., Yang, R., Xu, S., Zhang, Y., Li, C., Yang, S. ... Liang, G. (2017). Comprehensive analysis of aberrantly expressed microRNA profiles reveals potential biomarkers of human lung adenocarcinoma progression. Oncology Reports, 38, 2453-2463. https://doi.org/10.3892/or.2017.5880
MLA
Sui, J., Yang, R., Xu, S., Zhang, Y., Li, C., Yang, S., Yin, L., Pu, Y., Liang, G."Comprehensive analysis of aberrantly expressed microRNA profiles reveals potential biomarkers of human lung adenocarcinoma progression". Oncology Reports 38.4 (2017): 2453-2463.
Chicago
Sui, J., Yang, R., Xu, S., Zhang, Y., Li, C., Yang, S., Yin, L., Pu, Y., Liang, G."Comprehensive analysis of aberrantly expressed microRNA profiles reveals potential biomarkers of human lung adenocarcinoma progression". Oncology Reports 38, no. 4 (2017): 2453-2463. https://doi.org/10.3892/or.2017.5880