Suppression of LETM1 by siRNA inhibits cell proliferation and invasion of bladder cancer cells

  • Authors:
    • Bisheng Huang
    • Jingwei Zhang
    • Xiaolu Zhang
    • Chi Huang
    • Guanghui Hu
    • Saiyang Li
    • Tiancheng Xie
    • Mengnan Liu
    • Yunfei Xu
  • View Affiliations

  • Published online on: September 18, 2017     https://doi.org/10.3892/or.2017.5959
  • Pages: 2935-2940
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Abstract

The leucine zipper-EF-hand containing transmembrane protein 1 (LETM1) is highly expressed in many human malignancies and is correlated with poor prognosis. However, the function of LETM1 in bladder cancer still remains unknown. In the present study, we analyzed the expression levels of LETM1 in bladder cancer tissues and non-cancerous tissues as well as in four bladder cancer cell lines (T24, EJ, 5637 and J82) and a human bladder epithelial immortalized cell line SV-HUC-1. Small interfering RNA (siRNA) was employed to knockdown the expression of LETM1 in the T24 cells. The proliferation of T24 cells was significantly repressed as evaluated by CCK-8 assays. Transwell migration and invasion assays indicated that knockdown of LETM1 suppressed cell migration and invasion significantly. Flow cytometric analysis revealed that cells had accumulated at the S-phase when the expression of LETM1 was suppressed. Moreover, we found that several oncogenic proteins in the Wnt/β-catenin signaling pathway, namely β-catenin, cyclin D1 and c-Myc were significantly decreased by the LETM1 siRNA. Collectively, these results revealed that the knockdown of LETM1 exhibited tumor suppressive effects, possibly by controlling the downstream Wnt/β-catenin signaling pathway.
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November-2017
Volume 38 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Huang B, Zhang J, Zhang X, Huang C, Hu G, Li S, Xie T, Liu M and Xu Y: Suppression of LETM1 by siRNA inhibits cell proliferation and invasion of bladder cancer cells. Oncol Rep 38: 2935-2940, 2017
APA
Huang, B., Zhang, J., Zhang, X., Huang, C., Hu, G., Li, S. ... Xu, Y. (2017). Suppression of LETM1 by siRNA inhibits cell proliferation and invasion of bladder cancer cells. Oncology Reports, 38, 2935-2940. https://doi.org/10.3892/or.2017.5959
MLA
Huang, B., Zhang, J., Zhang, X., Huang, C., Hu, G., Li, S., Xie, T., Liu, M., Xu, Y."Suppression of LETM1 by siRNA inhibits cell proliferation and invasion of bladder cancer cells". Oncology Reports 38.5 (2017): 2935-2940.
Chicago
Huang, B., Zhang, J., Zhang, X., Huang, C., Hu, G., Li, S., Xie, T., Liu, M., Xu, Y."Suppression of LETM1 by siRNA inhibits cell proliferation and invasion of bladder cancer cells". Oncology Reports 38, no. 5 (2017): 2935-2940. https://doi.org/10.3892/or.2017.5959