Downregulation of ZNF278 arrests the cell cycle and decreases the proliferation of colorectal cancer cells via inhibition of the ERK/MAPK pathway

  • Authors:
    • Xiao-Qing Tian
    • Fang-Fang Guo
    • Dan-Feng Sun
    • Ying-Chao Wang
    • Li Yang
    • Sheng-Liang Chen
    • Jie Hong
    • Jing-Yuan Fang
  • View Affiliations

  • Published online on: October 12, 2017     https://doi.org/10.3892/or.2017.6031
  • Pages:3685-3692
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Abstract

Zinc finger protein 278 is a zinc finger transcription factor encoded on the 22q12.2 chromosome. Previous studies revealed that ZNF278 expression was significantly upregulated in colorectal cancer (CRC) tissue compared to adjacent non-tumor tissue. However, the expression and specific roles of ZNF278 in CRC remain unknown. ZNF278 expression was knocked down using specific siRNAs, which was confirmed by western blotting, and the effects of ZNF278 siRNAs on CRC cell proliferation were investigated. In addition, the effects of ZNF278 overexpression were confirmed by western blotting and cell proliferation assay. Correlations between ZNF278 and the ERK/MAPK pathway were also detected by western blotting. We found that ZNF278 knockdown significantly induced cell cycle arrest, resulting in cyclin D1/E1 downregulation and p21 upregulation. Moreover, we demonstrated that downregulation of ZNF278 decreased the proliferation of CRC cells via inhibition of the extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) pathway for the first time. In conclusion, ZNF278 played a prominent role in the pathogenesis of CRC, and promoted CRC cell proliferation via the ERK/MAPK pathway, suggesting that it may act as a potential target in the diagnosis or treatment of CRC.

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December 2017
Volume 38 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

2016 Impact Factor: 2.662
Ranked #31/217 Oncology
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APA
Tian, X., Guo, F., Sun, D., Wang, Y., Yang, L., Chen, S. ... Fang, J. (2017). Downregulation of ZNF278 arrests the cell cycle and decreases the proliferation of colorectal cancer cells via inhibition of the ERK/MAPK pathway. Oncology Reports, 38, 3685-3692. https://doi.org/10.3892/or.2017.6031
MLA
Tian, X., Guo, F., Sun, D., Wang, Y., Yang, L., Chen, S., Hong, J., Fang, J."Downregulation of ZNF278 arrests the cell cycle and decreases the proliferation of colorectal cancer cells via inhibition of the ERK/MAPK pathway". Oncology Reports 38.6 (2017): 3685-3692.
Chicago
Tian, X., Guo, F., Sun, D., Wang, Y., Yang, L., Chen, S., Hong, J., Fang, J."Downregulation of ZNF278 arrests the cell cycle and decreases the proliferation of colorectal cancer cells via inhibition of the ERK/MAPK pathway". Oncology Reports 38, no. 6 (2017): 3685-3692. https://doi.org/10.3892/or.2017.6031