CPNE1 silencing inhibits the proliferation, invasion and migration of human osteosarcoma cells

Jiang,Zhenhuan; Jiang,Jiannong; Zhao,Bizeng; Yang,Huilin; Wang,Yunliang; Guo,Shang; Deng,Youping; Lu,Deyi; Ma,Tieliang; Wang,Hongwei; Wang,Jinzhi

doi:10.3892/or.2017.6128

CPNE1 silencing inhibits the proliferation, invasion and migration of human osteosarcoma cells

Authors:
- Zhenhuan Jiang
- Jiannong Jiang
- Bizeng Zhao
- Huilin Yang
- Yunliang Wang
- Shang Guo
- Youping Deng
- Deyi Lu
- Tieliang Ma
- Hongwei Wang
- Jinzhi Wang
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Affiliations: Department of Orthopaedics, Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China, Department of Orthopedics, Affiliated Sixth People's Hospital of Shanghai JiaoTong University, Shanghai 20023, P.R. China, Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215200, P.R. China, Department of Neurology, The 148 Central Hospital of PLA, Zibo, Shangdong 255000, P.R. China, Bioinformatics Core, Department of Complementary and Integrative Medicine, University of Hawaii John A. Burns School of Medicine, Honolulu, HI 96813, USA, Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60607, USA, Central Laboratory, Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China, Shanghai Realgen Biotech Inc., Pudong New District, Shanghai 203215, P.R. China, Department of Cell Biology, School of Medicine, Soochow University, Suzhou, Jiangsu 215007, P.R. China
Published online on: December 4, 2017 https://doi.org/10.3892/or.2017.6128
Pages: 643-650

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Abstract

Osteosarcoma (OS) is the most common primary malignancy of the bone affecting children and adolescents. Copine 1 (CPNE1) is a highly conserved calcium-dependent phospholipid-binding protein and may function in regulating signal transduction and membrane trafficking. In the present study, we investigated CPNE1 expression in osteosarcoma tissues and cells, and studied the effects of small interfering RNA (siRNA)-targeting CPNE1 on proliferation, metastasis and chemosensitivity of the osteosarcoma cells. The results demonstrated that CPNE1 was highly expressed in the osteosarcoma tissues and cell lines. Moreover, functional investigations confirmed that CPNE1 knockdown significantly inhibited cell proliferation, colony formation, invasion and metastasis in Saos-2 and HOS cells. Western blot analysis indicated that CPNE1 silencing downregulated the expression of many proteins associated with tumorigenesis and development, including Ras, MEK-1/2, WNT1, β-catenin, cyclin A1, IRAK2 and cIAP2. In addition, CPNE1 downregulation enhanced the sensitivity of Saos-2 cells towards cisplatin and adriamycin. The present study provides deep insight into the clinical use of lentiviral-mediated CPNE1 silencing for osteosarcoma therapy.

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