ClC-3 chloride channel protein induces G1 arrest in hepatocellular carcinoma Hep3B cells

  • Authors:
    • Rui Wang
    • Bijun Kang
    • Rendong Hu
    • Youwai Huang
    • Zixi Qin
    • Jichen Du
    • Xi Lin
  • View Affiliations

  • Published online on: May 3, 2018     https://doi.org/10.3892/or.2018.6416
  • Pages: 472-478
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Abstract

ClC-3 is a type of chloride channel that has multiple functions in tumorigenesis and tumor growth, and can be blocked by DIDS (4,4'-diisothiocyanostilbene-2,2'-disulfonic acid). In the present study, we found that DIDS inhibited the proliferation of Hep3B hepatocellular carcinoma (HCC) cells in a concentration-dependent manner. More in-depth research demonstrated that DIDS downregulated the protein expression levels of cyclin D1 and cyclin E, which are key proteins of the G1 phase. Additionally, we found that ClC-3 siRNA transfection induced G1 arrest in the Hep3B cells, confirming that ClC-3 is involved in the DIDS-induced inhibition of Hep3B cells. Moreover, the level of α-fetoprotein (AFP), a negative prognostic indicator of HCC, was decreased after treatment with DIDS and ClC-3 siRNA. In conclusion, we demonstrated that ClC-3 can arrest the cell cycle at the G1 phase to inhibit cell proliferation, suggesting that ClC-3 has the potential to be a novel target for HCC therapy and potentially improve the prognosis of HCC patients.
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July-2018
Volume 40 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Wang R, Kang B, Hu R, Huang Y, Qin Z, Du J and Lin X: ClC-3 chloride channel protein induces G1 arrest in hepatocellular carcinoma Hep3B cells. Oncol Rep 40: 472-478, 2018.
APA
Wang, R., Kang, B., Hu, R., Huang, Y., Qin, Z., Du, J., & Lin, X. (2018). ClC-3 chloride channel protein induces G1 arrest in hepatocellular carcinoma Hep3B cells. Oncology Reports, 40, 472-478. https://doi.org/10.3892/or.2018.6416
MLA
Wang, R., Kang, B., Hu, R., Huang, Y., Qin, Z., Du, J., Lin, X."ClC-3 chloride channel protein induces G1 arrest in hepatocellular carcinoma Hep3B cells". Oncology Reports 40.1 (2018): 472-478.
Chicago
Wang, R., Kang, B., Hu, R., Huang, Y., Qin, Z., Du, J., Lin, X."ClC-3 chloride channel protein induces G1 arrest in hepatocellular carcinoma Hep3B cells". Oncology Reports 40, no. 1 (2018): 472-478. https://doi.org/10.3892/or.2018.6416