Open Access

PI3Kβ inhibitor AZD6482 exerts antiproliferative activity and induces apoptosis in human glioblastoma cells

  • Authors:
    • Peng-Fei Xu
    • Ji-An Yang
    • Jun-Hui Liu
    • Xue Yang
    • Jian-Ming Liao
    • Fan-En Yuan
    • Bao-Hui Liu
    • Qian-Xue Chen
  • View Affiliations

  • Published online on: November 2, 2018     https://doi.org/10.3892/or.2018.6845
  • Pages: 125-132
  • Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Glioblastoma is the most common type of primary brain tumour in adults, and its pathogenesis is particularly complicated. Among the many possible mechanisms underlying its pathogenesis, hyperactivation of the PI3K/Akt pathway is essential to the occurrence and development of glioma through the loss of PTEN or somatic activating mutations in PIK3CA. In the present study, we investigated the effect of the PI3Kβ inhibitor AZD6482 on glioma cells. The CCK-8 assay showed dose-dependent cytotoxicity in glioma cell lines treated with AZD6482. Additionally, AZD6482 treatment was found to significantly induce apoptosis and cell cycle arrest as detected using flow cytometry. Moreover, as shown using western blot analysis, the levels of p-AKT, p-GSK-3β, Bcl-2, and cyclin D1 were decreased after AZD6482 treatment. In addition, we found that AZD6482 inhibited the migration and invasion of glioma cells as detected by wound healing and Transwell invasion assays. Taken together, our findings indicate that AZD6482 exerts an antitumour effect by inhibiting proliferation and inducing apoptosis in human glioma cells. AZD6482 may be applied as an adjuvant therapy to improve the therapeutic efficacy of glioblastoma treatment.
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January-2019
Volume 41 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Xu P, Yang J, Liu J, Yang X, Liao J, Yuan F, Liu B and Chen Q: PI3Kβ inhibitor AZD6482 exerts antiproliferative activity and induces apoptosis in human glioblastoma cells. Oncol Rep 41: 125-132, 2019
APA
Xu, P., Yang, J., Liu, J., Yang, X., Liao, J., Yuan, F. ... Chen, Q. (2019). PI3Kβ inhibitor AZD6482 exerts antiproliferative activity and induces apoptosis in human glioblastoma cells. Oncology Reports, 41, 125-132. https://doi.org/10.3892/or.2018.6845
MLA
Xu, P., Yang, J., Liu, J., Yang, X., Liao, J., Yuan, F., Liu, B., Chen, Q."PI3Kβ inhibitor AZD6482 exerts antiproliferative activity and induces apoptosis in human glioblastoma cells". Oncology Reports 41.1 (2019): 125-132.
Chicago
Xu, P., Yang, J., Liu, J., Yang, X., Liao, J., Yuan, F., Liu, B., Chen, Q."PI3Kβ inhibitor AZD6482 exerts antiproliferative activity and induces apoptosis in human glioblastoma cells". Oncology Reports 41, no. 1 (2019): 125-132. https://doi.org/10.3892/or.2018.6845