Histamine is involved in the regulation of collagen content in cultured heart myofibroblasts via H2, H3 and H4 histamine receptors

  • Authors:
    • Lucyna Piera
    • Jacek Szymański
    • Marlena Juszczak
    • Jacek Drobnik
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  • Published online on: July 5, 2021     https://doi.org/10.3892/br.2021.1447
  • Article Number: 71
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Abstract

Histamine is involved in the regulation of collagen metabolism during healing following a myocardial infarction; however, its effects on the intact heart tissue is unknown. The aim of the present study was to determine whether histamine may influence collagen content in cells isolated from intact heart, and to identify the histamine receptor involved in the regulation of collagen deposition. Cells were isolated from intact rat hearts and subjected to identification by flow cytometry. The effects of histamine and its receptor agonists and antagonists were investigated. The heart cells were found to be actin, desmin and vimentin positive. Histamine (used at a concentrations of 1x10‑10‑1x10‑5 M) increased collagen content within the culture and increased the expression of α1 chain of the procollagen type III gene. The H2, H3 and H4 receptor inhibitors ranitidine, ciproxifan and JNJ 7777120 blocked the effect of histamine on collagen content. All tested histamine receptor agonists, viz. 2‑pyridylethylamine dihydrochloride (H1 receptor agonist), amthamine dihydrobromide (H2 receptor agonist), imetit (H3 receptor agonist) and 4‑methylhistamine hydrochloride (H4 receptor agonist), elevated collagen content within the heart myofibroblast cultures. The cells isolated from the intact heart were identified as myofibroblasts. Thus, the results of the present study showed that histamine augmented collagen content in the heart myofibroblast culture by activation of three histamine receptors (H2, H3 and H4). The effect of the amine was also dependent on the activation of collagen type III gene expression.
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August-2021
Volume 15 Issue 2

Print ISSN: 2049-9434
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Spandidos Publications style
Piera L, Szymański J, Juszczak M and Drobnik J: Histamine is involved in the regulation of collagen content in cultured heart myofibroblasts via H<sub>2</sub>, H<sub>3</sub> and H<sub>4</sub> histamine receptors. Biomed Rep 15: 71, 2021
APA
Piera, L., Szymański, J., Juszczak, M., & Drobnik, J. (2021). Histamine is involved in the regulation of collagen content in cultured heart myofibroblasts via H<sub>2</sub>, H<sub>3</sub> and H<sub>4</sub> histamine receptors. Biomedical Reports, 15, 71. https://doi.org/10.3892/br.2021.1447
MLA
Piera, L., Szymański, J., Juszczak, M., Drobnik, J."Histamine is involved in the regulation of collagen content in cultured heart myofibroblasts via H<sub>2</sub>, H<sub>3</sub> and H<sub>4</sub> histamine receptors". Biomedical Reports 15.2 (2021): 71.
Chicago
Piera, L., Szymański, J., Juszczak, M., Drobnik, J."Histamine is involved in the regulation of collagen content in cultured heart myofibroblasts via H<sub>2</sub>, H<sub>3</sub> and H<sub>4</sub> histamine receptors". Biomedical Reports 15, no. 2 (2021): 71. https://doi.org/10.3892/br.2021.1447