<em>Rhizopus oryzae</em> AM16; a new hyperactive L‑asparaginase producer: Semi solid‑state production and anticancer activity of the partially purified protein

Othman,Sarah I.; Mekawey,Amal A.I.; El‑Metwally,Mohammad M.; Gabr,Sami A.; Alwaele,Maha Abdulla; Al Fassam,Haifa; Abo‑Eleneen,Rasha; Allam,Ahmed A.; Saber,Wesameldin I.A.

doi:10.3892/br.2022.1498

Rhizopus oryzae AM16; a new hyperactive L‑asparaginase producer: Semi solid‑state production and anticancer activity of the partially purified protein

Authors:
- Sarah I. Othman
- Amal A.I. Mekawey
- Mohammad M. El‑Metwally
- Sami A. Gabr
- Maha Abdulla Alwaele
- Haifa Al Fassam
- Rasha Abo‑Eleneen
- Ahmed A. Allam
- Wesameldin I.A. Saber
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Affiliations: Department of Biology, Faculty of Science, Princess Nourah Bint Abdulrahman University, Riyadh 11671, Saudi Arabia, The Regional Center of Mycology and Biotechnology, Al‑Azhar University, Cairo 11765, Egypt, Botany and Microbiology Department, Faculty of Science, Damanhour University, Damanhour 22511, Egypt, Department of Anatomy and Embryology, Faculty of Medicine, Mansoura University, Mansoura 35511, Egypt, Zoology Department, Faculty of Science, Beni‑Suef University, Beni‑Suef 62511, Egypt, Microbial Activity Unit, Department of Microbiology, Soils, Water and Environment Research Institute, Agricultural Research Center, Giza 12619, Egypt
Published online on: January 14, 2022 https://doi.org/10.3892/br.2022.1498
Article Number: 15
Copyright: © Othman et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The demand for L‑asparaginase is predicted to increase several fold in the future due to its potential clinical applications in the treatment of lymphoid system malignancies and leukemia. Thus identifying suitable sources of production should be considered high priority. Fungi are valuable organisms as they are able to convert what would be considered ‘useless’ materials into materials that have potential value. The present study provides a proof of concept of production of a new hyperactive L‑asparaginase producer (Rhizopus oryzae AM16), which was successfully isolated and sequentially optimized using a semi solid‑state fermentation method with a simple and cheap medium produced from wheat bran (WB). The fungus was able to produce an appreciable amount of the enzyme (2,875.9 U) after 8 days of incubation under 85.7% moisture, in the presence of magnesium nitrate (5.0 mg N/mg nitrogen per gram of dry WB) at pH 5.8. Testing the anticancer activity confirmed the ability of the resultant enzyme to inhibit the growth of various types of cancer cells (HepG2, MCF‑7, HCT and A549). The IC₅₀ values of the dialyzed enzyme were lower than that of the crude product. Thus, this newly identified and purified L‑asparaginase may be a promising anticancer drug.

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