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Effects of pine needle essential oil on melanin synthesis in B16F10 cells and its mechanism

  • Authors:
    • Suyuan Lü
    • Bingqing Shang
    • Guolong Liu
    • Luyan Sun
    • Qiu Wu
    • Yue Geng
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Food Nutrition and Safety of SDNU, Provincial Key Laboratory of Animal Resistant Biology, College of Life Science, Shandong Normal University, Jinan, Shandong 250358, P.R. China
    Copyright: © Lü et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 133
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    Published online on: June 6, 2025
       https://doi.org/10.3892/br.2025.2011
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Abstract

The present study aimed to investigate the melanin synthesis effect of pine needle essential oil (PNEO) extracted using microwave‑assisted extraction on α‑MSH‑induced B16F10 melanoma cells and its molecular mechanism of action. Cell Counting Kit‑8 was used to measure the safe concentration of B16F10 cells after 24 and 48 h post‑treatment. Tyrosinase (TYR) activity assay was used to examine intracellular enzyme viability; western blotting (WB) and reverse transcription‑quantitative (RT‑qPCR) assays were utilized to assess the transcription and expression of genes and proteins associated with melanogenesis. Relevant targets in the cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA) signaling pathway were verified by the addition of target inhibitors or activators. Results showed that PNEO decreased the levels of melanin and the activity of TYR in B16F10 cells. RT‑qPCR and WB results showed that PNEO downregulated the expression of melanogenesis‑related genes and proteins such as microphthalmia‑associated transcription factor, TYR, TYR‑related protein‑1 and melanocortin 1 receptor, and reduced the levels of phosphorylated PKA and phosphorylated cyclic‑AMP response binding protein. These results suggested that the inhibitory effect of PNEO on melanin production may be related to the cAMP/PKA pathway. Verification through the addition of target inhibitors or activators confirmed that PNEO regulates melanin synthesis and TYR activity through the cAMP/PKA signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Lü S, Shang B, Liu G, Sun L, Wu Q and Geng Y: Effects of pine needle essential oil on melanin synthesis in B16F10 cells and its mechanism. Biomed Rep 23: 133, 2025.
APA
Lü, S., Shang, B., Liu, G., Sun, L., Wu, Q., & Geng, Y. (2025). Effects of pine needle essential oil on melanin synthesis in B16F10 cells and its mechanism. Biomedical Reports, 23, 133. https://doi.org/10.3892/br.2025.2011
MLA
Lü, S., Shang, B., Liu, G., Sun, L., Wu, Q., Geng, Y."Effects of pine needle essential oil on melanin synthesis in B16F10 cells and its mechanism". Biomedical Reports 23.2 (2025): 133.
Chicago
Lü, S., Shang, B., Liu, G., Sun, L., Wu, Q., Geng, Y."Effects of pine needle essential oil on melanin synthesis in B16F10 cells and its mechanism". Biomedical Reports 23, no. 2 (2025): 133. https://doi.org/10.3892/br.2025.2011
Copy and paste a formatted citation
x
Spandidos Publications style
Lü S, Shang B, Liu G, Sun L, Wu Q and Geng Y: Effects of pine needle essential oil on melanin synthesis in B16F10 cells and its mechanism. Biomed Rep 23: 133, 2025.
APA
Lü, S., Shang, B., Liu, G., Sun, L., Wu, Q., & Geng, Y. (2025). Effects of pine needle essential oil on melanin synthesis in B16F10 cells and its mechanism. Biomedical Reports, 23, 133. https://doi.org/10.3892/br.2025.2011
MLA
Lü, S., Shang, B., Liu, G., Sun, L., Wu, Q., Geng, Y."Effects of pine needle essential oil on melanin synthesis in B16F10 cells and its mechanism". Biomedical Reports 23.2 (2025): 133.
Chicago
Lü, S., Shang, B., Liu, G., Sun, L., Wu, Q., Geng, Y."Effects of pine needle essential oil on melanin synthesis in B16F10 cells and its mechanism". Biomedical Reports 23, no. 2 (2025): 133. https://doi.org/10.3892/br.2025.2011
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