Analysis of differential gene expression by bead-based fiber‑optic array in growth-hormone-secreting pituitary adenomas

Jiang,Zhiquan; Gui,Songbo; Zhang,Yazhuo

doi:10.3892/etm.2010.137

Analysis of differential gene expression by bead-based fiber‑optic array in growth-hormone-secreting pituitary adenomas

Authors:
- Zhiquan Jiang
- Songbo Gui
- Yazhuo Zhang
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Affiliations: Capital Medical University, 100069 Beijing, P.R. China, Department of Neurosurgery, Beijing Tiantan Hospital, 100050 Beijing, P.R. China, Beijing Neurosurgical Institute, 100050 Beijing, P.R. China
Published online on: July 21, 2010 https://doi.org/10.3892/etm.2010.137
Pages: 905-910

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Abstract

Growth-hormone-secreting pituitary adenomas (GHomas) account for approximately 20% of all pituitary neoplasms. However, the pathogenesis of GHomas remains to be elucidated. To explore the possible pathogenesis of GHomas, we used bead-based fiber-optic arrays to examine the gene expression in five GHomas and compared them to three healthy pituitaries. Four differentially expressed genes were chosen randomly for validation by quantitative real-time reverse transcription-polymerase chain reaction. We then performed pathway analysis on the identified differentially expressed genes using the Kyoto Encyclopedia of Genes and Genomes. Array analysis showed significant increases in the expression of 353 genes and 206 expressed sequence tags (ESTs) and decreases in 565 genes and 29 ESTs. Bioinformatic analysis showed that the genes HIGD1B, HOXB2, ANGPT2, HPGD and BTG2 may play an important role in the tumorigenesis and progression of GHomas. Pathway analysis showed that the wingless-type signaling pathway and extracellular-matrix receptor interactions may play a key role in the tumorigenesis and progression of GHomas. Our data suggested that there are numerous aberrantly expressed genes and pathways involved in the pathogenesis of GHomas. Bead-based fiber-optic arrays combined with pathway analysis of differentially expressed genes appear to be a valid method for investigating the pathogenesis of tumors.

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