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Article

Analysis of differential gene expression by bead-based fiber‑optic array in growth-hormone-secreting pituitary adenomas

  • Authors:
    • Zhiquan Jiang
    • Songbo Gui
    • Yazhuo Zhang
  • View Affiliations / Copyright

    Affiliations: Capital Medical University, 100069 Beijing, P.R. China, Department of Neurosurgery, Beijing Tiantan Hospital, 100050 Beijing, P.R. China, Beijing Neurosurgical Institute, 100050 Beijing, P.R. China
  • Pages: 905-910
    |
    Published online on: July 21, 2010
       https://doi.org/10.3892/etm.2010.137
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Abstract

Growth-hormone-secreting pituitary adenomas (GHomas) account for approximately 20% of all pituitary neoplasms. However, the pathogenesis of GHomas remains to be elucidated. To explore the possible pathogenesis of GHomas, we used bead-based fiber-optic arrays to examine the gene expression in five GHomas and compared them to three healthy pituitaries. Four differentially expressed genes were chosen randomly for validation by quantitative real-time reverse transcription-polymerase chain reaction. We then performed pathway analysis on the identified differentially expressed genes using the Kyoto Encyclopedia of Genes and Genomes. Array analysis showed significant increases in the expression of 353 genes and 206 expressed sequence tags (ESTs) and decreases in 565 genes and 29 ESTs. Bioinformatic analysis showed that the genes HIGD1B, HOXB2, ANGPT2, HPGD and BTG2 may play an important role in the tumorigenesis and progression of GHomas. Pathway analysis showed that the wingless-type signaling pathway and extracellular-matrix receptor interactions may play a key role in the tumorigenesis and progression of GHomas. Our data suggested that there are numerous aberrantly expressed genes and pathways involved in the pathogenesis of GHomas. Bead-based fiber-optic arrays combined with pathway analysis of differentially expressed genes appear to be a valid method for investigating the pathogenesis of tumors.
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1. 

Pollock BE, Jacob JT, Brown PD and Nippoldt TB: Radiosurgery of growth hormone-producing pituitary adenomas: factors associated with biochemical remission. J Neurosurg. 106:833–838. 2007. View Article : Google Scholar : PubMed/NCBI

2. 

Rajasoorya C, Holdaway IM, Wrightson P and Scott DJ: Ibbertson HK determinants of clinical outcome and survival in acromegaly. Clin Endocrinol. 41:95–102. 1994. View Article : Google Scholar : PubMed/NCBI

3. 

Beauregard C, Truong U, Hardy J and Serri O: Long-term outcome and mortality after transsphenoidal adenomectomy for acromegaly. Clin Endocrinol. 58:86–91. 2003. View Article : Google Scholar : PubMed/NCBI

4. 

Herman V, Fagin J, Gonsky R, Kovacs K and Melmed S: Clonal origin of pituitary adenomas. J Clin Endocrinol Metab. 71:1427–1433. 1990. View Article : Google Scholar : PubMed/NCBI

5. 

Farrell WE and Clayton RN: Molecular genetics of pituitary tumours. Trends Endocrinol Metab. 9:20–26. 1998. View Article : Google Scholar : PubMed/NCBI

6. 

Ezzat S, Kontogeorgos G, Redelmeier DA, Horvath E, Harris AG and Kovacs K: In vivo responsiveness of morphological variants of growth hormone-producing pituitary adenomas to octreotide. Eur J Endocrinol. 133:686–690. 1995. View Article : Google Scholar : PubMed/NCBI

7. 

Karga HJ, Alexander JM, Hedley-Whyte ET, Klibanski A and Jameson JL: Ras mutations in human pituitary tumors. J Clin Endocrinol Metab. 74:914–919. 1992. View Article : Google Scholar : PubMed/NCBI

8. 

Asa SL and Ezzat S: The pathogenesis of pituitary tumors. Annu Rev Pathol. 4:97–126. 2009. View Article : Google Scholar : PubMed/NCBI

9. 

Mao S and Dong G: Discovery of highly differentiative gene groups from microarray gene expression data using the gene club approach. J Bioinform Comput Biol. 3:1263–1280. 2005. View Article : Google Scholar : PubMed/NCBI

10. 

Evans CO, Young AN, Brown MR, Brat DJ, Parks JS, Neish AS and Oyesiku NM: Novel patterns of gene expression in pituitary adenomas identified by complementary deoxyribonucleic acid microarrays and quantitative reverse transcription-polymerase chain reaction. J Clin Endocrinol Metab. 86:3097–3107. 2001.

11. 

Morris DG, Musat M, Czirjak S, Hanzely Z, Lillington DM, Korbonits M and Grossman AB: Differential gene expression in pituitary adenomas by oligonucleotide array analysis. Eur J Endocrinol. 153:143–151. 2005. View Article : Google Scholar : PubMed/NCBI

12. 

Ellestad LE, Carre W, Muchow M, Jenkins SA, Wang X, Cogburn LA and Porter TE: Gene expression profiling during cellular differentiation in the embryonic pituitary gland using cDNA microarrays. Physiol Genomics. 25:414–425. 2006. View Article : Google Scholar : PubMed/NCBI

13. 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

14. 

Denko N, Schindler C, Koong A, Laderoute K, Green C and Giaccia A: Epigenetic regulation of gene expression in cervical cancer cells by the tumor microenvironment. Clin Cancer Res. 6:480–487. 2000.PubMed/NCBI

15. 

Lewis MT: Homeobox genes in mammary gland development and neoplasia. Breast Cancer Res. 2:158–169. 2000. View Article : Google Scholar : PubMed/NCBI

16. 

Lappin TR, Grier DG, Thompson A and Halliday HL: HOX genes: seductive science, mysterious mechanisms. Ulster Med J. 75:23–31. 2006.PubMed/NCBI

17. 

Wang HL, Deng CS, Lin J, Pan DY, Zou ZY and Zhou XY: Expression of angiopoietin-2 is correlated with vascularization and tumor size in human colorectal adenocarcinoma. Tohoku J Exp Med. 213:33–40. 2007. View Article : Google Scholar : PubMed/NCBI

18. 

Nag S, Nourhaghighi N, Venugopalan R, Asa SL and Stewart DJ: Angiopoietins are expressed in the normal rat pituitary gland. Endocr Pathol. 16:67–73. 2005. View Article : Google Scholar : PubMed/NCBI

19. 

Hu B, Guo P, Fang Q, et al: Angiopoietin-2 induces human glioma invasion through the activation of matrix metalloprotease-2. Proc Natl Acad Sci USA. 100:8904–8909. 2003. View Article : Google Scholar : PubMed/NCBI

20. 

Jang TJ, Ji YS and Jung KH: Decreased expression of 15-hydroxyprostaglandin dehydrogenase in gastric carcinomas. Yonsei Med J. 49:917–922. 2008. View Article : Google Scholar : PubMed/NCBI

21. 

Coggins KG, Latour A, Nguyen MS, Audoly L, Coffman TM and Koller BH: Metabolism of PGE2 by prostaglandin dehydrogenase is essential for remodeling the ductus arteriosus. Nat Med. 8:91–92. 2002. View Article : Google Scholar : PubMed/NCBI

22. 

Wolf I, O’Kelly J, Rubinek T, et al: 15-hydroxyprostaglandin dehydrogenase is a tumor suppressor of human breast cancer. Cancer Res. 66:7818–7823. 2006. View Article : Google Scholar : PubMed/NCBI

23. 

Rouault JP, Falette N, Guehenneux F, et al: Identification of BTG2, an antiproliferative p53-dependent component of the DNA damage cellular response pathway. Nat Genet. 14:482–486. 1996. View Article : Google Scholar : PubMed/NCBI

24. 

Cortes U, Moyret-Lalle C, Falette N, et al: BTG gene expression in the p53-dependent and -independent cellular response to DNA damage. Mol Carcinog. 27:57–64. 2000. View Article : Google Scholar : PubMed/NCBI

25. 

Farioli-Vecchioli S, Tanori M, Micheli L, et al: Inhibition of medulloblastoma tumorigenesis by the antiproliferative and prodifferentiative gene PC3. FASEB J. 21:2215–2225. 2007. View Article : Google Scholar : PubMed/NCBI

26. 

Subramanian A, Tamayo P, Mootha VK, et al: Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles. Proc Natl Acad Sci USA. 102:15545–15550. 2005. View Article : Google Scholar : PubMed/NCBI

27. 

Karim R, Tse G, Putti T, Scolyer R and Lee S: The significance of the Wnt pathway in the pathology of human cancers. Pathology. 36:120–128. 2004. View Article : Google Scholar : PubMed/NCBI

28. 

Jones SE and Jomary C: Secreted Frizzled-related proteins: searching for relationships and patterns. Bioessays. 24:811–820. 2002. View Article : Google Scholar : PubMed/NCBI

29. 

Elston MS, Gill AJ, Conaglen JV, et al: Wnt pathway inhibitors are strongly down-regulated in pituitary tumors. Endocrinology. 149:1235–1242. 2008. View Article : Google Scholar : PubMed/NCBI

30. 

Danen EH and Yamada KM: Fibronectin, integrins, and growth control. J Cell Physiol. 189:1–13. 2001. View Article : Google Scholar : PubMed/NCBI

31. 

Huet C, Pisselet C, Mandon-Pepin B, Monget P and Monniaux D: Extracellular matrix regulates ovine granulosa cell survival, proliferation and steroidogenesis: relationships between cell shape and function. J Endocrinol. 169:347–360. 2001. View Article : Google Scholar

32. 

Paez-Pereda M, Kuchenbauer F, Arzt E and Stalla GK: Regulation of pituitary hormones and cell proliferation by components of the extracellular matrix. Braz J Med Biol Res. 38:1487–1494. 2005. View Article : Google Scholar : PubMed/NCBI

33. 

Gerger A, Hofmann G, Langsenlehner U, et al: Integrin alpha-2 and beta-3 gene polymorphisms and colorectal cancer risk. Int J Colorectal Dis. 24:159–163. 2009. View Article : Google Scholar : PubMed/NCBI

34. 

Sawhney RS, Cookson MM, Omar Y, Hauser J and Brattain MG: Integrin alpha2-mediated ERK and calpain activation play a critical role in cell adhesion and motility via focal adhesion kinase signaling: identification of a novel signaling pathway. J Biol Chem. 281:8497–8510. 2006. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Jiang Z, Gui S and Zhang Y: Analysis of differential gene expression by bead-based fiber‑optic array in growth-hormone-secreting pituitary adenomas. Exp Ther Med 1: 905-910, 2010.
APA
Jiang, Z., Gui, S., & Zhang, Y. (2010). Analysis of differential gene expression by bead-based fiber‑optic array in growth-hormone-secreting pituitary adenomas. Experimental and Therapeutic Medicine, 1, 905-910. https://doi.org/10.3892/etm.2010.137
MLA
Jiang, Z., Gui, S., Zhang, Y."Analysis of differential gene expression by bead-based fiber‑optic array in growth-hormone-secreting pituitary adenomas". Experimental and Therapeutic Medicine 1.5 (2010): 905-910.
Chicago
Jiang, Z., Gui, S., Zhang, Y."Analysis of differential gene expression by bead-based fiber‑optic array in growth-hormone-secreting pituitary adenomas". Experimental and Therapeutic Medicine 1, no. 5 (2010): 905-910. https://doi.org/10.3892/etm.2010.137
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang Z, Gui S and Zhang Y: Analysis of differential gene expression by bead-based fiber‑optic array in growth-hormone-secreting pituitary adenomas. Exp Ther Med 1: 905-910, 2010.
APA
Jiang, Z., Gui, S., & Zhang, Y. (2010). Analysis of differential gene expression by bead-based fiber‑optic array in growth-hormone-secreting pituitary adenomas. Experimental and Therapeutic Medicine, 1, 905-910. https://doi.org/10.3892/etm.2010.137
MLA
Jiang, Z., Gui, S., Zhang, Y."Analysis of differential gene expression by bead-based fiber‑optic array in growth-hormone-secreting pituitary adenomas". Experimental and Therapeutic Medicine 1.5 (2010): 905-910.
Chicago
Jiang, Z., Gui, S., Zhang, Y."Analysis of differential gene expression by bead-based fiber‑optic array in growth-hormone-secreting pituitary adenomas". Experimental and Therapeutic Medicine 1, no. 5 (2010): 905-910. https://doi.org/10.3892/etm.2010.137
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