Up-regulation of microRNAs, miR21 and miR23a in human liver cancer cells treated with Coptidis rhizoma aqueous extract

  • Authors:
    • Meifen Zhu
    • Ning Wang
    • Sai-Wah Tsao
    • Man-Fung Yuen
    • Yigang Feng
    • Thomas S.K. Wan
    • Kwan Man
    • Yibin Feng
  • View Affiliations

  • Published online on: November 19, 2010     https://doi.org/10.3892/etm.2010.164
  • Pages: 27-32
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Abstract

Coptidis rhizoma (CR; Huanglian in Chinese) has been used for the treatment of cancer in Chinese medicine, and recent studies have supported its use in cancer therapy. MicroRNAs (miRNAs) play an important role in the pathophysiology of human cancers. We examined alterations in the miRNA profile of hepatocellular carcinoma (HCC) cells after treatment with coptidis rhizoma aqueous extract (CRAE). An on-chip microarray method was used to detect alterations in the expression profile of miRNAs in human HCC MHCC97-L cells after exposure to 175 µg/ml CRAE. Altered expression of several miRNAs was detected in the MHCC97-L cells after treatment with 175 µg/ml CRAE. The microarray results were validated by quantitative real-time PCR (qRT-PCR). Consistent results were obtained; qRT-PCR confirmed that both miR-21 and miR-23a were significantly up-regulated. TargetScan and PicTar microRNA databases were used to predict the possible target genes of the altered miRNAs. The results showed that the altered miRNAs after CRAE treatment may serve as markers for the therapy of liver cancer. To the best of our knowledge, this is the first report on the up-regulation of miRNAs, miR21 and miR23a in human liver cancer cells treated with CRAE. Our results suggest that CRAE targets miR-21 and miR-23a in liver cancer cells supporting the potential application of CRAE in the treatment of HCC.
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January-February 2011
Volume 2 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhu M, Wang N, Tsao S, Yuen M, Feng Y, Wan TS, Man K and Feng Y: Up-regulation of microRNAs, miR21 and miR23a in human liver cancer cells treated with Coptidis rhizoma aqueous extract. Exp Ther Med 2: 27-32, 2011.
APA
Zhu, M., Wang, N., Tsao, S., Yuen, M., Feng, Y., Wan, T.S. ... Feng, Y. (2011). Up-regulation of microRNAs, miR21 and miR23a in human liver cancer cells treated with Coptidis rhizoma aqueous extract. Experimental and Therapeutic Medicine, 2, 27-32. https://doi.org/10.3892/etm.2010.164
MLA
Zhu, M., Wang, N., Tsao, S., Yuen, M., Feng, Y., Wan, T. S., Man, K., Feng, Y."Up-regulation of microRNAs, miR21 and miR23a in human liver cancer cells treated with Coptidis rhizoma aqueous extract". Experimental and Therapeutic Medicine 2.1 (2011): 27-32.
Chicago
Zhu, M., Wang, N., Tsao, S., Yuen, M., Feng, Y., Wan, T. S., Man, K., Feng, Y."Up-regulation of microRNAs, miR21 and miR23a in human liver cancer cells treated with Coptidis rhizoma aqueous extract". Experimental and Therapeutic Medicine 2, no. 1 (2011): 27-32. https://doi.org/10.3892/etm.2010.164