Antioxidant activity and antiapoptotic effect of Asparagus racemosus root extracts in human lung epithelial H460 cells

  • Authors:
    • Lalana Kongkaneramit
    • Wasu Witoonsaridsilp
    • Penchom Peungvicha
    • Kornkanok Ingkaninan
    • Neti Waranuch
    • Narong Sarisuta
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  • Published online on: December 2, 2010     https://doi.org/10.3892/etm.2010.172
  • Pages: 143-148
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Abstract

The present study examined the antioxidant activity and protective effect of extracts from Asparagus racemosus roots against Lipofectamine-induced apoptosis. Five fractions from a successive extraction process ranging from non-polar to more polar solvents were obtained. The total saponin content as a marker in terms of diosgenin equivalent value of the root extracts was found to be in the range of 240-420 µg/mg extract, with higher values for the ethanol and aqueous fractions. The antioxidant activity measured using the DPPH method in terms of mean effective concentration (EC50) of the aqueous fraction was found to be 600 µg/ml as compared to 1.5 µg/ml of ascorbic acid. It is proposed that Asparagus racemosus root extracts effectively inhibit Lipofectamine-induced apoptosis by their protective effect, and may serve as an advantageous alternative option for gene delivery.
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January-February 2011
Volume 2 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Kongkaneramit L, Witoonsaridsilp W, Peungvicha P, Ingkaninan K, Waranuch N and Sarisuta N: Antioxidant activity and antiapoptotic effect of Asparagus racemosus root extracts in human lung epithelial H460 cells. Exp Ther Med 2: 143-148, 2011
APA
Kongkaneramit, L., Witoonsaridsilp, W., Peungvicha, P., Ingkaninan, K., Waranuch, N., & Sarisuta, N. (2011). Antioxidant activity and antiapoptotic effect of Asparagus racemosus root extracts in human lung epithelial H460 cells. Experimental and Therapeutic Medicine, 2, 143-148. https://doi.org/10.3892/etm.2010.172
MLA
Kongkaneramit, L., Witoonsaridsilp, W., Peungvicha, P., Ingkaninan, K., Waranuch, N., Sarisuta, N."Antioxidant activity and antiapoptotic effect of Asparagus racemosus root extracts in human lung epithelial H460 cells". Experimental and Therapeutic Medicine 2.1 (2011): 143-148.
Chicago
Kongkaneramit, L., Witoonsaridsilp, W., Peungvicha, P., Ingkaninan, K., Waranuch, N., Sarisuta, N."Antioxidant activity and antiapoptotic effect of Asparagus racemosus root extracts in human lung epithelial H460 cells". Experimental and Therapeutic Medicine 2, no. 1 (2011): 143-148. https://doi.org/10.3892/etm.2010.172