Ostrich produce cross-reactive neutralization antibodies against pandemic influenza virus A/H1N1 following immunization with a seasonal influenza vaccine

Adachi,Kazuhide; Takama,Kentaro; Tsukamoto,Masaya; Inai,Marie; Handharyani,Ekowati; Hiroi,Satoshi; Tsukamoto,Yasuhiro

doi:10.3892/etm.2010.180

January-February 2011 Volume 2 Issue 1

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January-February 2011 Volume 2 Issue 1

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Article

Ostrich produce cross-reactive neutralization antibodies against pandemic influenza virus A/H1N1 following immunization with a seasonal influenza vaccine

Authors:
- Kazuhide Adachi
- Kentaro Takama
- Masaya Tsukamoto
- Marie Inai
- Ekowati Handharyani
- Satoshi Hiroi
- Yasuhiro Tsukamoto
View Affiliations / Copyright

Affiliations: Department of Animal Hygiene, Graduate School of Environmental and Biological Sciences, Kyoto Prefecture University, Shimogamo, Kyoto 606-8522, Japan, Faculty of Veterinary Medicine, Bogor Agricultural University, JL. Agatis, Kampus IPB Darmaga, Bogor 16680, Indonesia, Department of Virology, Osaka Prefectural Institute of Public Health, Higashinari-ku, Osaka City, Osaka 537-0025, Japan
Pages: 41-45
|
Published online on: December 2, 2010

https://doi.org/10.3892/etm.2010.180
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Abstract

An outbreak of influenza in 2009 was found to be caused by a novel strain of influenza virus designated as pandemic influenza A/H1N1 2009. Vaccination with recent seasonal influenza vaccines induced little or no cross-reactive antibody response to the pandemic influenza virus A/H1N1 2009 in any age group in human populations. Accordingly, most people had low immunity against this pathogen, thus resulting in the worldwide spread of the infection to produce a so-called ‘pandemic’. This report presents the important finding that ostrich eggs generate cross-reactive antibodies to the pandemic influenza virus A/H1N1 following immunization of female ostrich with a seasonal influenza vaccine. This simple method produced a large amount of antibodies against influenza viruses by one female ostrich. An enzyme-linked immunosorbent assay (ELISA) and immunocytochemistry indicated that the ostrich antibodies possessed strong cross-reactivity to the pandemic A/H1N1 as well as to the seasonal A/H1N1, A/H3N2 and B viruses. The hemaggregation activities of erythrocytes induced by this pandemic strain were also inhibited by the ostrich antibodies. In addition, the cytopathological effects of infection with a pandemic virus on MDCK cells were clearly inhibited in co-cultures with the ostrich antibodies, thereby indicating the neutralization of viral infectivity in the cells. In conclusion, cross-reactive neutralization antibodies against pandemic influenza virus A/H1N1 2009 were successfully generated in ostrich eggs produced by females immunized with seasonal influenza viral vaccine.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Ostrich produce cross-reactive neutralization antibodies against pandemic influenza virus A/H1N1 following immunization with a seasonal influenza vaccine

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Abstract

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